Physicochemical characterization of a novel graphene-based magnetic resonance imaging contrast agent
| Autor: | Sayan Mullick Chowdhury, Terry M. Button, Jimmy Toussaint, Gaurav Lalwani, William Moore, Kenneth R. Shroyer, Shruti Kanakia, Tanuf Tembulkar, Balaji Sitharaman |
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| Rok vydání: | 2013 |
| Předmět: |
Materials science
Chemical Phenomena Gadolinium Biophysics Analytical chemistry Pharmaceutical Science chemistry.chemical_element Contrast Media Bioengineering Manganese physicochemical properties law.invention Biomaterials chemistry.chemical_compound In vivo law International Journal of Nanomedicine Drug Discovery preclinical Thermostability Original Research Graphene Phantoms Imaging Organic Chemistry graphene Dextrans General Medicine contrast agent Magnetic Resonance Imaging Nanostructures Partition coefficient Dextran chemistry dextran relaxivity Thermogravimetry Graphite Protein adsorption Nuclear chemistry |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Shruti Kanakia,1 Jimmy D Toussaint,1 Sayan Mullick Chowdhury,1 Gaurav Lalwanim,1 Tanuf Tembulkar,1 Terry Button,1,2 Kenneth R Shroyer,3 William Moore,2 Balaji Sitharaman11Department of Biomedical Engineering, 2Department of Radiology, 3Department of Pathology, Stony Brook University, Stony Brook, NY, USAAbstract: We report the synthesis and characterization of a novel carbon nanostructure-based magnetic resonance imaging contrast agent (MRI CA); graphene nanoplatelets intercalated with manganese (Mn2+) ions, functionalized with dextran (GNP-Dex); and the in vitro assessment of its essential preclinical physicochemical properties: osmolality, viscosity, partition coefficient, protein binding, thermostability, histamine release, and relaxivity. The results indicate that, at concentrations between 0.1 and 100.0 mg/mL, the GNP-Dex formulations are hydrophilic, highly soluble, and stable in deionized water, as well as iso-osmolar (upon addition of mannitol) and iso-viscous to blood. At potential steady-state equilibrium concentrations in blood (0.1–10.0 mg/mL), the thermostability, protein-binding, and histamine-release studies indicate that the GNP-Dex formulations are thermally stable (with no Mn2+ ion dissociation), do not allow non-specific protein adsorption, and elicit negligible allergic response. The r1 relaxivity of GNP-Dex was 92 mM-1s-1 (per-Mn2+ ion, 22 MHz proton Larmor frequency); ~20- to 30-fold greater than that of clinical gadolinium (Gd3+)- and Mn2+-based MRI CAs. The results open avenues for preclinical in vivo safety and efficacy studies with GNP-Dex toward its development as a clinical MRI CA.Keywords: manganese, dextran, preclinical, physicochemical properties, relaxivity, graphene, magnetic resonance imaging, contrast agent |
| Databáze: | OpenAIRE |
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