Use of Nucleic Acid Amplification Testing for Diagnosis of Anorectal Sexually Transmitted Infections
| Autor: | Tracy Zamborsky, Abi Jett, Tracy E. Campbell, Sharon L. Hillier, Ingrid Macio, Ross D. Cranston, Lisa A. Cosentino |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Microbiology (medical) Adolescent Sexual Behavior Sexually Transmitted Diseases Rectum Chlamydia trachomatis Mycoplasma genitalium urologic and male genital diseases medicine.disease_cause Sensitivity and Specificity Microbiology Young Adult Trichomonas vaginalis medicine Humans biology Multiple displacement amplification Bacteriology Nucleic acid amplification technique Middle Aged biology.organism_classification Virology female genital diseases and pregnancy complications Neisseria gonorrhoeae United States medicine.anatomical_structure Molecular Diagnostic Techniques Nucleic acid Female Nucleic Acid Amplification Techniques |
| Zdroj: | Journal of Clinical Microbiology. 50:2005-2008 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.00185-12 |
| Popis: | Nucleic acid amplification testing (NAAT) has become the preferred method to detect Chlamydia trachomatis and Neisseria gonorrhoeae , but no commercial tests are cleared by the U.S. Food and Drug Administration for use with rectal swab samples. This study evaluated the performance of strand displacement amplification (SDA) and transcription-mediated amplification (TMA) to detect C. trachomatis and N. gonorrhoeae and to determine if TMA could also detect Mycoplasma genitalium and Trichomonas vaginalis in men and women reporting a history of receptive anal intercourse. Discordant results between the NAATs were reevaluated using the Aptima CT or Aptima GC assay, each of which targets primers other than those targeted by the Aptima Combo 2 (AC2) assay, as the confirmatory test. Of 497 evaluable participants, 41 (8.2%) were positive for C. trachomatis , 21 (4.2%) were positive for N. gonorrhoeae , 26 (5.2%) were positive for T. vaginalis , and 47 (9.5%) were positive for M. genitalium . The sensitivity and specificity of the C. trachomatis test were 100% and 99.8% for AC2 and 56.1% and 100% for SDA, respectively. The sensitivity and specificity of the N. gonorrhoeae test were 100% and 100% for AC2 and 76.2% and 100% for SDA, respectively, while culture was only 23.8% sensitive. Of the 114 participants who had a positive result for any of the four infectious agents, 16 were positive for two pathogens and 3 were positive for three pathogens. These data suggest that rectal infection is common and that the AC2 is superior to SDA for the detection of C. trachomatis and N. gonorrhoeae from rectal swab samples. |
| Databáze: | OpenAIRE |
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