Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses
| Autor: | Hanieh Falahati, Christine Prater, Raul Ramos, Sumana Raychaudhuri, Shuo Li, Shigeki Watanabe, Tomas M. Bartol, Grant F. Kusick, Jing Wang, Eric Hosy, Marisa M Brockmann, Sarah Syed, Stephen A. Lee |
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| Přispěvatelé: | Johns Hopkins University (JHU), Texas Tech University Health Sciences Center, Texas Tech University [Lubbock] (TTU), Yale University School of Medicine, Brandeis University, The Salk Institute for Biological Studies, Interdisciplinary Institute for Neuroscience (IINS), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Science [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Models Neurological Primary Cell Culture Action Potentials Glutamic Acid General Physics and Astronomy AMPA receptor Hippocampal formation Hippocampus Receptors N-Methyl-D-Aspartate Synaptic Transmission Article Exocytosis General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences chemistry.chemical_compound Spatio-Temporal Analysis 0302 clinical medicine mental disorders Synaptic vesicle exocytosis Animals Computer Simulation Receptors AMPA Active zone Neurotransmitter Cells Cultured Neurons Multidisciplinary Chemistry musculoskeletal neural and ocular physiology Depolarization General Chemistry Microscopy Electron 030104 developmental biology nervous system Astrocytes Synapses Biophysics NMDA receptor Female biological phenomena cell phenomena and immunity 030217 neurology & neurosurgery psychological phenomena and processes |
| Zdroj: | Nature Communications Nature Communications, Nature Publishing Group, 2021, 12, pp.677. ⟨10.1038/s41467-021-21004-x⟩ Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021) |
| ISSN: | 2041-1723 |
| Popis: | Neurotransmitter is released synchronously and asynchronously following an action potential. Our recent study indicates that the release sites of these two phases are segregated within an active zone, with asynchronous release sites enriched near the center in mouse hippocampal synapses. Here we demonstrate that synchronous and asynchronous release sites are aligned with AMPA receptor and NMDA receptor clusters, respectively. Computational simulations indicate that this spatial and temporal arrangement of release can lead to maximal membrane depolarization through AMPA receptors, alleviating the pore-blocking magnesium leading to greater activation of NMDA receptors. Together, these results suggest that release sites are likely organized to activate NMDA receptors efficiently. Action potentials induce synchronous and asynchronous release of neurotransmitters. Here, the authors show that the synchronous and asynchronous release sites are aligned with AMPARs and NMDARs, respectively, in mouse hippocampal synapses. This organization allows efficient activation of NMDARs. |
| Databáze: | OpenAIRE |
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