Biomineralization of Recombinant Peptide Scaffolds: Interplay among Chemistry, Architecture, and Mechanics
| Autor: | Kendell M. Pawelec, Sebastiaan G. J. M. Kluijtmans |
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| Rok vydání: | 2017 |
| Předmět: |
Scaffold
biology Cell growth Chemistry 0206 medical engineering Cell Biomedical Engineering Osteoblast 02 engineering and technology Mechanics 021001 nanoscience & nanotechnology 020601 biomedical engineering Mineralization (biology) Biomaterials medicine.anatomical_structure Tissue engineering Osteocalcin biology.protein medicine 0210 nano-technology Biomineralization |
| Zdroj: | ACS Biomaterials Science & Engineering. 3:1100-1108 |
| ISSN: | 2373-9878 |
| Popis: | Biomineralized scaffolds are an attractive option for bone tissue engineering, being similar to native bone. However, optimization is difficult, due to the complex interplay among architecture, chemistry, and mechanics. Utilizing biomimetic nucleation, linear mineralized scaffolds were created from a collagen type I based recombinant peptide (RCP). Osteoblast mineralization was assessed, in response to changes in scaffold architecture, hydroxyapatite (HA) content, and mechanics. Changes in scaffold pore size (150-450 μm) had little effect on mRNA levels but influenced cell proliferation, achieving a balance between nutrient diffusion and surface area for cell attachment at 300 μm. Increasing the scaffold mechanical strength, from 2.9 to 5.2 kPa, enhanced the expression of osteocalcin, a late marker of mineralization. Further addition of HA, up to 20 wt %, increased osteoblast mineralization, without altering the compressive modulus. Thus, it was shown that architectural cues influence cellular proliferation, while the scaffold chemistry and mechanics independently contribute to gene expression. |
| Databáze: | OpenAIRE |
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