Homozygous deletion of the activin A receptor, type IB gene is associated with an aggressive cancer phenotype in pancreatic cancer
| Autor: | Hiroki Sakamoto, Hidetoshi Hayashi, Akihiko Ito, Masatoshi Kudo, Kazuko Sakai, Kazuto Nishio, Yasuo Kodera, Masayuki Kitano, Shuta Tomida, Masato Terashima, Marco A. De Velasco, Yosuke Togashi, Yoshihiko Fujita |
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| Rok vydání: | 2014 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Male Cancer Research animal structures Cell Survival Type IB Gene Dosage Mice Nude Smad2 Protein Adenocarcinoma Biology SMAD4 Gene dosage Activin signal Mice Activin A receptor Cell Line Tumor Pancreatic cancer medicine Animals Humans Neoplasm Invasiveness RNA Small Interfering Activin type 2 receptors Cell Proliferation Smad4 Protein Regulation of gene expression Research Homozygote Activin receptor Middle Aged medicine.disease Survival Analysis Gene Expression Regulation Neoplastic Pancreatic Neoplasms Phenotype Oncology embryonic structures Cancer research Molecular Medicine Female Signal transduction Activin Receptors Type I Gene Deletion Neoplasm Transplantation Signal Transduction Transforming growth factor |
| Zdroj: | Molecular Cancer |
| ISSN: | 1476-4598 |
| DOI: | 10.1186/1476-4598-13-126 |
| Popis: | Background Transforming growth factor, beta (TGFB) signal is considered to be a tumor suppressive pathway based on the frequent genomic deletion of the SMAD4 gene in pancreatic cancer (PC); however; the role of the activin signal, which also belongs to the TGFB superfamily, remains largely unclear. Methods and results We found a homozygous deletion of the activin A receptor, type IB (ACVR1B) gene in 2 out of 8 PC cell lines using array-comparative genomic hybridization, and the absence of ACVR1B mRNA and protein expression was confirmed in these 2 cell lines. Activin A stimulation inhibited cellular growth and increased the phosphorylation level of SMAD2 and the expression level of p21CIP1/WAF1 in the Sui66 cell line (wild-type ACVR1B and SMAD4 genes) but not in the Sui68 cell line (homozygous deletion of ACVR1B gene). Stable ACVR1B-knockdown using short hairpin RNA cancelled the effects of activin A on the cellular growth of the PC cell lines. In addition, ACVR1B-knockdown significantly enhanced the cellular growth and colony formation abilities, compared with controls. In a xenograft study, ACVR1B-knockdown resulted in a significantly elevated level of tumorigenesis and a larger tumor volume, compared with the control. Furthermore, in clinical samples, 6 of the 29 PC samples (20.7%) carried a deletion of the ACVR1B gene, while 10 of the 29 samples (34.5%) carried a deletion of the SMAD4 gene. Of note, 5 of the 6 samples with a deletion of the ACVR1B gene also had a deletion of the SMAD4 gene. Conclusion We identified a homozygous deletion of the ACVR1B gene in PC cell lines and clinical samples and proposed that the deletion of the ACVR1B gene may mediate an aggressive cancer phenotype in PC. Our findings provide novel insight into the role of the activin signal in PC. |
| Databáze: | OpenAIRE |
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