Assessment of Rhodopseudomonas sphaeroides chromatophore membrane asymmetry through bilateral antiserum adsorption studies
Autor: | D. E. Mallon, M. L P Collins, Robert Niederman |
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Rok vydání: | 1980 |
Předmět: |
L-Lactate Dehydrogenase
Membrane Proteins Dehydrogenase Bacterial Chromatophores Rhodobacter sphaeroides Periplasmic space Spheroplast Biology Microbiology Chromatophore Transmembrane protein chemistry.chemical_compound Membrane Bacterial Proteins chemistry Biochemistry Cytoplasm NADH NADPH Oxidoreductases Bacteriochlorophyll Bacteriochlorophylls Immunoelectrophoresis Two-Dimensional Molecular Biology Research Article |
Zdroj: | Journal of Bacteriology. 143:221-230 |
ISSN: | 1098-5530 0021-9193 |
DOI: | 10.1128/jb.143.1.221-230.1980 |
Popis: | The asymmetric structure of the Rhodopseudomonas sphaeroides chromatophore membrane was examined in detail by crossed immunoelectrophoresis techniques. Because these methods are quantitative and allow increased resolution and sensitivity, it was possible to analyze simultaneously the relative transmembrane distribution of a number of previously identified antigenic components. This was demonstrated by analysis of immunoglobulin samples that were adsorbed by preincubation with either isolated chromatophores or osmotically protected spheroplasts. The photochemical reaction center, the light-harvesting bacteriochlorophyll a-protein complex, the L-lactate dehydrogenase, and reduced nicotinamide adenine dinucleotide dehydrogenase (EC 1.6.99.3) were found to be exposed on the chromatophore surface (cytoplasmic aspect of the membrane within the cell). Other antigenic components were found to be exposed on the surface of spheroplasts (periplasmic aspect of the in vivo chromatophore membrane). Antigens with determinants expressed on both sides of the chromatophore membrane were also identified. Charge shift crossed immunoelectrophoresis confirmed the suggested amphiphilic character of the pigment-protein complexes and identified several additional amphiphilic membrane components. |
Databáze: | OpenAIRE |
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