Pre- and Posttransplant Monitoring of Alloantibodies by Complement-Dependent Cytotoxicity and Luminex Methodologies in Liver Transplantation
| Autor: | Jorge Kalil, Maria Cristina Ribeiro de Castro, N. Panajotopoulos, Francisco Antonio Monteiro, Helcio Rodrigues, Sérgio Mies, M. Paredes, P. Massarolo |
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| Rok vydání: | 2012 |
| Předmět: |
Cytotoxicity
Immunologic Graft Rejection Graft dysfunction Time Factors T-Lymphocytes medicine.medical_treatment Human leukocyte antigen Liver transplantation Fluorescence Microsphere HLA Antigens Isoantibodies Monitoring Immunologic Predictive Value of Tests medicine Humans Cytotoxicity Complement Activation ANTICORPOS B-Lymphocytes Transplantation biology business.industry Histocompatibility Testing Cytotoxicity Tests Immunologic equipment and supplies Complement-dependent cytotoxicity Liver Transplantation respiratory tract diseases Treatment Outcome Histocompatibility Immunology biology.protein Surgery Antibody business Antibody screening Biomarkers |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0041-1345 |
| DOI: | 10.1016/j.transproceed.2012.07.005 |
| Popis: | Background This study evaluated the influence of circulating anti-HLA antibodies on outcomes of 97 liver allografts from deceased donors. Methods Human leukocyte antigen (HLA) antibody screening was performed by both complement-dependent cytotoxicity (CDC) and multiparameter Luminex microsphere-based assays (Luminex assay). Results The agreements between T- and B- cell CDC and Luminex assays were 67% and 77% for pre- and posttransplant specimens, respectively. Graft dysfunction was not associated with either positive pretransplant CDC or Luminex panel-reactive antibody (PRA) values. Likewise, positive posttransplant T- or B- cell CDC PRA values were not associated with graft dysfunction. In contrast, posttransplant Luminex PRA values were significantly higher among patients with graft dysfunction compared with subjects with good outcomes ( P = .017). Conclusion Posttransplant monitoring of HLA antibodies with Luminex methodology allowed identification of patients at high-risk for poor graft outcomes. |
| Databáze: | OpenAIRE |
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