GTP gamma S-induced Ca2+ activated Cl- currents: its stable induction by Gq alpha overexpression in Xenopus oocytes
| Autor: | Muneshige Kaibara, Yasuhito Uezono, Yoshiyuki Doi, Kohtaro Taniyama, Osamu Murasaki, Yoshihisa Nagase |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Microinjections Cations Divalent Xenopus Guanosine Biology chemistry.chemical_compound Chloride Channels Internal medicine medicine Animals Pharmacology Oocyte biology.organism_classification Heterotrimeric GTP-Binding Proteins Cell biology Rats Endocrinology medicine.anatomical_structure chemistry Guanosine 5'-O-(3-Thiotriphosphate) Oocytes GTP-Binding Protein alpha Subunits Gq-G11 Calcium Female |
| Zdroj: | Japanese journal of pharmacology. 86(2) |
| ISSN: | 0021-5198 |
| Popis: | In native Xenopus oocytes, injection of guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS) (30 mM, 5 nl) did not induce Cl- current in 11 out of 22 oocytes. Injection of increased concentration of GTPgammaS (100 mM, 5 nl) into the oocytes induced Cl- currents in 16 out of 17 oocytes; however, the size of the induced currents was extremely varied. In oocytes overexpressing Gq alpha, GTPgammaS (30 mM, 5 nl) faithfully evoked Ca2+-activated Cl- currents. These results indicate that heterogeneous expression of Gq alpha in Xenopus oocytes provides a useful system for studying the functional roles of Gq alpha in regulating cellular events. |
| Databáze: | OpenAIRE |
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