Immunochemical studies of the lipopolysaccharide O-specific polysaccharide of Hafnia alvei PCM 1199 related to H. alvei PCM 1205
| Autor: | Małgorzata Mieszała, Georgy V. Zatonsky, Ewa Katzenellenbogen, Maria Bogulska, Nina A. Kocharova, Yuriy A. Knirel, Aleksander S. Shashkov, Elzbieta Romanowska, Andrzej Gamian |
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| Rok vydání: | 1998 |
| Předmět: |
chemistry.chemical_classification
Teichoic acid Chromatography biology Stereochemistry Molecular Sequence Data Carbohydrates O Antigens Glycosidic bond Polysaccharide biology.organism_classification Methylation Biochemistry Enterobacteriaceae Teichoic Acids chemistry.chemical_compound Residue (chemistry) Carbohydrate Sequence Species Specificity chemistry Carbohydrate Conformation Glycerol Acid hydrolysis Carbohydrate conformation |
| Zdroj: | European Journal of Biochemistry. 251:980-985 |
| ISSN: | 1432-1033 0014-2956 |
| Popis: | On the basis of chemical analyses and NMR spectroscopic studies, it was found that the O-specific polysaccharide (O-PS) isolated from the Hafnia alvei PCM 1199 lipopolysaccharide (LPS) is a glycerol teichoic-acid-like polymer having a repeating unit of the following structure: [structure in text] where Qui4NAc is 4-acetamido-4,6-dideoxyglucose and O-acetylation at both positions is non-stoichiometric. The glycosidic linkage of the lateral beta-D-GlcpNAc residue is acid-labile and cleaved from the O-PS during mild acid hydrolysis or dephosphorylation with 48% hydrofluoric acid. Comparative analysis revealed that the structure of the H. alvei PCM 1199 O-PS is similar to that of H. alvei PCM 1205, which differs in the presence of an additional lateral alpha-D-Glcp residue and the position of one of the O-acetyl groups only. Accordingly, serological tests revealed a high degree of serological similarity between LPSs and O-PSs of the two H. alvei strains. |
| Databáze: | OpenAIRE |
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