Lipoxin A4 promotes autophagy and inhibits overactivation of macrophage inflammasome activity induced by Pg LPS
| Autor: | Jiayi Liu, Wenjing Geng, Xiujuan Xiong, Fengjun Xi, Jie Zhao, Xiangqun Xu, Xu Huang, Xiaodong Kuang, Kefei Wan, Kailei Guo |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Medicine (General) autophagy Lipoxin a4 Lipopolysaccharide Inflammasomes Biochemistry Inflammasome Microbiology Pre-Clinical Research Report Mice 03 medical and health sciences chemistry.chemical_compound R5-920 0302 clinical medicine medicine Animals Macrophage Pg LPS LXA4 Porphyromonas gingivalis biology business.industry Macrophages Biochemistry (medical) Autophagy NF-kappa B Cell Biology General Medicine biology.organism_classification Lipoxins 030104 developmental biology chemistry 030220 oncology & carcinogenesis business medicine.drug |
| Zdroj: | The Journal of International Medical Research Journal of International Medical Research, Vol 49 (2021) |
| ISSN: | 1473-2300 0300-0605 |
| DOI: | 10.1177/0300060520981259 |
| Popis: | Objective To explore the role of lipoxin A4 (LXA4) on inflammasome and inflammatory activity in macrophages activated by Porphyromonas gingivalis lipopolysaccharide (PgLPS) one of the major causative agents of chronic periodontitis. Methods The mouse macrophage cell line RAW264.7 was used to produce an activated inflammation model. Markers of inflammasome and inflammatory activity and autophagy were assessed by ELISA, reverse transcription polymerase chain reaction (RT-PCR), and Western blot assay. Results Markers of inflammasome activity, inflammation and autophagy increased with Pg LPS concentration. They also increased with increasing exposure to Pg LPS up to 12h but decreased at 24h. However, markers of autophagy increased. Phosphorylated NF-κBp65 decreased with LXA4, which was similar to results obtained with the autophagy inducer, rapamycin. Conclusions LXA4 promoted autophagy and inhibited activation of inflammasomes and inflammation markers in macrophage inflammation induced by PgLPS and this action was linked to the phosphorylation of NF-κB. |
| Databáze: | OpenAIRE |
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