Dose-Related Effects of Hyperoxia on the Lung Inflammatory Response in Septic Rats
| Autor: | Ophir Lavon, Nitza Lahat, Haim Bitterman, Vera Brod, Yuval Cavari, Hanni Menn-Josephy, Miriam David, Bat-Chen Amit-Cohen, Michal A. Rahat, Dan Waisman, Doron Rimar |
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| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Inflammation Video microscopy Hyperoxia Critical Care and Intensive Care Medicine Rats Sprague-Dawley Sepsis Pulmonary sequestration Internal medicine Leukocytes medicine Animals L-Selectin Lung Nitrites Dose-Response Relationship Drug biology CD11 Antigens Tumor Necrosis Factor-alpha Chemistry Pneumonia medicine.disease Rats Oxygen Disease Models Animal Endocrinology medicine.anatomical_structure Gene Expression Regulation Emergency Medicine biology.protein L-selectin Tumor necrosis factor alpha medicine.symptom |
| Zdroj: | Shock. 37:95-102 |
| ISSN: | 1073-2322 |
| DOI: | 10.1097/shk.0b013e3182356fc3 |
| Popis: | We evaluated the effects of hyperoxia on pulmonary inflammatory changes in sepsis induced by cecal ligation and puncture (CLP) in rats. Seven groups were studied: sham-operated rats breathing air for 20 or 48 h; CLP breathing air for 20 or 48 h; and CLP + 100% oxygen for 20 h, or 70% oxygen for 48 h, or 100% oxygen intermittently (6 h/d) for 48 h. Video microscopy was used to monitor lung macromolecular leak, microvascular flow velocity, and shear rates, and lung morphometry was used for leukocyte infiltration and solid tissue area. Cell counts, tumor necrosis factor α, and nitrites were determined in peripheral blood and lung lavage fluid. Expression of adhesion molecules in blood leukocytes was evaluated by flow cytometry. Cecal ligation and puncture induced inflammation manifested in leukopenia, left shift, thrombocytopenia, increased expression of L selectin and CD11, increased serum and lavage fluid tumor necrosis factor α and leukocytes, and increased lung tissue area, macromolecular leak, and sequestration of leukocytes. Inhalation of 100% oxygen for 20 h increased nitrites (P < 0.01) and decreased leukocyte count in lavage fluid (P < 0.05) and attenuated lung macromolecular leak and changes in solid tissue area (P < 0.01). Inhalation of 70% oxygen (48 h) attenuated expression of adhesion molecules (P < 0.001) but failed to attenuate markers of lung inflammation. In contrast, intermittent 100% oxygen exerted favorable effects on markers of inflammation, attenuated leukocyte expression of L selectin and CD11 (P < 0.01), decreased pulmonary sequestration of leukocytes (P < 0.001), and ameliorated changes in macromolecular leak (P < 0.01) and lung solid tissue area (P < 0.05). Our data support the beneficial effects of safe subtoxic regimens of normobaric hyperoxia on the systemic and pulmonary inflammatory response following CLP. |
| Databáze: | OpenAIRE |
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