Use of the natural products from the leaves of the fruitfull tree Persea americana against Candida sp. biofilms using acrylic resin discs
| Autor: | Adryelle Idalina da Silva Alves, Franz de Assis Graciano dos Santos, Jacqueline Cosmo Andrade, Maria Flaviana Bezerra Morais-Braga, Melyna Chaves Leite-Andrade, Paulo Riceli Vasconcelos Ribeiro, Michellângelo Nunes, Débora Lima Sales, Rejane Pereira Neves, Henrique Douglas Melo Coutinho, Maria Audilene de Freitas |
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| Rok vydání: | 2020 |
| Předmět: |
Persea
Antifungal Agents Environmental Engineering 010504 meteorology & atmospheric sciences Acrylic Resins Microbial Sensitivity Tests 010501 environmental sciences 01 natural sciences Trees Minimum inhibitory concentration chemistry.chemical_compound Tandem Mass Spectrometry medicine Environmental Chemistry Food science Waste Management and Disposal Acrylic resin Candida 0105 earth and related environmental sciences Biological Products Ethanol biology Biofilm biochemical phenomena metabolism and nutrition biology.organism_classification Antimicrobial Pollution Plant Leaves chemistry Biofilms visual_art visual_art.visual_art_medium Formazan Fluconazole medicine.drug |
| Zdroj: | Science of The Total Environment. 703:134779 |
| ISSN: | 0048-9697 |
| Popis: | The search for natural substances such as plant extracts with antimicrobial properties has considerably increased, given that biofilms constitute a barrier against antifungal therapy, where these can be formed on any surface, such as acrylic resin prosthesis. The objective of this study was to identify the chemical composition of the Persea americana Mill. leaf ethanol extract (EEFPa) using the UPLC-QTOF-MS/MS technique, to verify its antifungal activity through a sensitivity test according to the conditions described in the documents in M27-A3 (CLSI, 2008) and M60 (CLSI, 2017), to induce biofilm formation in acrylic resin discs and quantify their formation using tetrazolium salt reduction (MTT), as well as to treat these with the extract and fluconazole. Ten of the twelve compounds present in the extract were identified. In the sensitivity test the lowest minimum inhibitory concentration observed was 512 μg/mL, while fluconazole concentrations ranged from 64 to 1 μg/mL. During biofilm induction, all the isolates were able to form biofilms within 48 h. During biofilm treatment, the extract was less effective at biofilm reduction than Fluconazole. The EEFPa showed significant antifungal activity against some of the strains in this study, however the extract showed lower effect when compared to fluconazole against the biofilm formation. |
| Databáze: | OpenAIRE |
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