Interaction of roses with a biotrophic and a hemibiotrophic leaf pathogen leads to differences in defense transcriptome activation
| Autor: | Ina Menz, Helgard Kaufmann, Marcus Linde, Thomas Debener, Helena Sophia Domes, Enzo Neu |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Plant Science
phytohormone Transcriptome immunology plant protein plant gene Plant Growth Regulators Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik) Gene Expression Regulation Plant WRKY57 protein Arabidopsis pathogenicity genetics transcription factor Black spot rose Plant Proteins Genetics WRKY genes Chitinases High-Throughput Nucleotide Sequencing General Medicine gene expression regulation Diplocarpon rosae PR genes Plant disease ddc:580 chitinase Host-Pathogen Interactions Salicylic Acid Podosphaera pannosa signal transduction Host–pathogen interaction Biology Genes Plant Rosa high throughput sequencing Powdery mildew Ascomycota gene expression profiling flavonoid Cell wall modification host pathogen interaction plant disease plant leaf Plant Diseases Flavonoids Arabidopsis protein Arabidopsis Proteins microbiology Pathogen-Associated Molecular Pattern Molecules High-throughput qPCR Immunity biology.organism_classification pathogen associated molecular pattern WRKY protein domain Ascomycetes Plant Leaves MACE analysis Agronomy and Crop Science metabolism Transcription Factors |
| Zdroj: | Plant Molecular Biology 99 (2019), Nr. 43589 |
| DOI: | 10.15488/10451 |
| Popis: | Key message: Transcriptomic analysis resulted in the upregulation of the genes related to common defense mechanisms for black spot and the downregulation of the genes related to photosynthesis and cell wall modification for powdery mildew. Abstract: Plant pathogenic fungi successfully colonize their hosts by manipulating the host defense mechanisms, which is accompanied by major transcriptome changes in the host. To characterize compatible plant pathogen interactions at early stages of infection by the obligate biotrophic fungus Podosphaera pannosa, which causes powdery mildew, and the hemibiotrophic fungus Diplocarpon rosae, which causes black spot, we analyzed changes in the leaf transcriptome after the inoculation of detached rose leaves with each pathogen. In addition, we analyzed differences in the transcriptomic changes inflicted by both pathogens as a first step to characterize specific infection strategies. Transcriptomic changes were analyzed using next-generation sequencing based on the massive analysis of cDNA ends approach, which was validated using high-throughput qPCR. We identified a large number of differentially regulated genes. A common set of the differentially regulated genes comprised of pathogenesis-related (PR) genes, such as of PR10 homologs, chitinases and defense-related transcription factors, such as various WRKY genes, indicating a conserved but insufficient PTI [pathogen associated molecular pattern (PAMP) triggered immunity] reaction. Surprisingly, most of the differentially regulated genes were specific to the interactions with either P. pannosa or D. rosae. Specific regulation in response to D. rosae was detected for genes from the phenylpropanoid and flavonoid pathways and for individual PR genes, such as paralogs of PR1 and PR5, and other factors of the salicylic acid signaling pathway. Differently, inoculation with P. pannosa leads in addition to the general pathogen response to a downregulation of genes related to photosynthesis and cell wall modification. © 2019, The Author(s). |
| Databáze: | OpenAIRE |
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