Is the composition of exhaled breath condensate a key to explain the course of COVID-19 in children?
| Autor: | Alicja Krejner-Bienias, Patrycja Nejman-Gryz, Rafał Krenke, Katarzyna Grzela, Katarzyna Górska, Ewa Kobylska, Tomasz Grzela, Dorota Stadnik |
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| Rok vydání: | 2021 |
| Předmět: |
exhaled breath condensate
Original Paper medicine.medical_specialty Kawasaki-like syndrome Coronavirus disease 2019 (COVID-19) Chemistry business.industry Biological activity Dermatology Pharmacology In vitro respiratory tract diseases Endothelial stem cell Cell culture SARS-CoV-2/COVID-19 medicine Cytotoxic T cell Immunology and Allergy MTT assay Composition (visual arts) Exhaled breath condensate Intensive care medicine business Composition (language) |
| Zdroj: | Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii |
| ISSN: | 1642-395X |
| DOI: | 10.5114/ada.2020.97395 |
| Popis: | Introduction The relative resistance of children to severe course of the novel coronavirus infection remains unclear. We hypothesized that there might be a link between this phenomenon and observation from our previous studies concerning an inhibitory or cytotoxic effect of exhaled breath condensate (EBC) on endothelial cell cultures in children. Aim Since we could not find any data on the similar effect caused by EBC in adults, the aim of our study was to evaluate and compare the biological activity of EBC in adults and children in an experimental in vitro model. Furthermore, in order to identify a putative agent responsible for these properties of EBC in children, we attempted to analyse the composition of selected EBC samples. Material and methods The influence of EBC samples on metabolic activity of endothelial cell line C-166 was assessed using colorimetric tetrazolium salt reduction assay (MTT assay). Selected EBC samples were fractionated using size exclusion chromatography and subjected to mass spectrometry analysis. Results Exhaled breath condensates in healthy children, but not in adults, revealed a cytotoxic effect on in vitro cell cultures. This effect was most significant in condensate fraction, which contained a prominent 4.8 kDa peak in the mass spectra. Conclusions Breath condensates of healthy children contain the factor which reveals the inhibitory/cytotoxic effect on endothelial cell cultures. Although the physiological role of this agent remains unclear, its identification may potentially be useful in ongoing research on SARS-CoV-2/COVID-19. |
| Databáze: | OpenAIRE |
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