Alteration of serum/glucocorticoid regulated kinase-1 (sgk-1) gene expression in rat hippocampus after transient global ischemia
| Autor: | Satoshi Asai, Akiko Murata, Megumi Sugahara-Kobayashi, Yayoi Nishida, Yasuo Takahashi, Toshihito Nagata |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Central nervous system Ischemia Hippocampus In situ hybridization Biology Protein Serine-Threonine Kinases Gene Expression Regulation Enzymologic Immediate-Early Proteins Cellular and Molecular Neuroscience Internal medicine Gene expression medicine Reaction Time Animals RNA Messenger Rats Wistar Molecular Biology Gene Glucocorticoids Kinase Pyramidal Cells Nuclear Proteins medicine.disease Rats Up-Regulation Disease Models Animal Endocrinology medicine.anatomical_structure Glucose Ischemic Attack Transient Reperfusion Injury Glucocorticoid medicine.drug |
| Zdroj: | Brain research. Molecular brain research. 123(1-2) |
| ISSN: | 0169-328X |
| Popis: | Expression of the serum/glucocorticoid regulated kinase-1 (sgk-1) gene has been reported to be induced by various stress stimuli such as hyper- or hypo-osmotic stress, UV irradiation, and heat shock stress; however, its association with global ischemia in the brain has not been studied. Using high-density oligonucleotide array analysis, we found that the sgk-1 gene was one of the genes showing alteration of expression in the rat hippocampus during 1-4 h of reperfusion after 10 min of transient global cerebral ischemia. Using TaqMan RT-PCR analysis, we confirmed an increased level of sgk-1 gene expression with statistical significance in the rat hippocampus at 2 h of reperfusion after 10 min of transient global cerebral ischemia. Using in situ hybridization (ISH) analysis, the increased level of sgk-1 gene expression was found to localize in pyramidal cells of CA2 and CA3 regions of the hippocampus after 2 h of reperfusion. These results provide an insight into the alterations of sgk-1 gene expression in the rat hippocampus after transient global cerebral ischemia. |
| Databáze: | OpenAIRE |
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