Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides
| Autor: | Jayakumar Singh Bondili, Chandra Sekhar Bokka, Venkata Bala Sai Chaitanya Thunuguntla, Ganesh Kumar Veeramachaneni, Janakiram Bobbillapati |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
In silico Population peptide mapping Peptide medicine.disease_cause Immunoglobulin E Article 03 medical and health sciences Epitopes Mice Profilins 0302 clinical medicine Allergen Antigen medicine Animals Computer Simulation profilin education Sorghum pollen allergen modeling Th1 and Th2 cytokine chemistry.chemical_classification education.field_of_study lcsh:R5-920 biology General Medicine Disease Models Animal 030104 developmental biology 030228 respiratory system Profilin chemistry Biochemistry Docking (molecular) biology.protein lcsh:Medicine (General) |
| Zdroj: | Medicina Volume 55 Issue 5 Medicina, Vol 55, Iss 5, p 178 (2019) Medicina; Volume 55; Issue 5; Pages: 178 |
| ISSN: | 1010-660X |
| DOI: | 10.3390/medicina55050178 |
| Popis: | Background and objectives: Nearly 20&ndash 30% of the world&rsquo s population suffers from allergic rhinitis, among them 15% are progressing to asthma conditions. Sorghum bicolor profilin (Sorb PF), one of the panallergens, was identified, but the allergen specificity is not yet characterized. Materials and Methods: To map the antigenic determinants responsible for IgE binding, the present study is focused on in silico modeling, simulation of Sorb PF and docking of the Sorb PF peptides (PF1-6) against IgG and IgE, followed by in vivo evaluation of the peptides for its allergenicity in mice. Results: Peptide PF3 and PF4 displayed high docking G-scores (&minus 9.05) against IgE only. The mice sensitized with PF3 peptide showed increased levels of IL5, IL12, TNF-alpha, and GMCSF when compared to other peptides and controls, signifying a strong, Th2-based response. Concurrently, the Th1 pathway was inhibited by low levels of cytokine IL2, IFN-&gamma and IL-10 justifying the role of PF3 in allergenic IgE response. Conclusions: Based on the results of overlapping peptides PF3 and PF4, the N-terminal part of the PF3 peptide (TGQALVI) plays a crucial role in allergenic response of Sorghum profilin. |
| Databáze: | OpenAIRE |
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