Discrepancy in divergence of the mitochondrial and nuclear genomes of Drosophila teissieri and Drosophila yakuba
| Autor: | David R. Wolstenholme, Monique Monnerot, Michel Solignac |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Genetics
Mitochondrial DNA biology Base Sequence Molecular Sequence Data Restriction Mapping Nucleic acid sequence Genetic Variation biology.organism_classification DNA Mitochondrial Drosophila virilis Restriction site Restriction map Genes Drosophilidae Sequence Homology Nucleic Acid Transfer RNA Africa Animals Drosophila Molecular Biology Ecology Evolution Behavior and Systematics Phylogeny Drosophila yakuba |
| Zdroj: | Journal of molecular evolution. 30(6) |
| ISSN: | 0022-2844 |
| Popis: | Restriction sites were compared in the mitochondrial DNA (mtDNA) molecules from representatives of two closely related species of fruit flies: nine strains of Drosophila teissieri and eight strains of Drosophila yakuba. Nucleotide diversities among D. teissieri strains and among D. yakuba strains were 0.07% and 0.03%, respectively, and the nucleotide distance between the species was 0.22%. Also determined was the nucleotide sequence of a 2305-nucleotide pair (ntp) segment of the mtDNA molecule of D. teissieri that contains the noncoding adenine + thymine (A + T)-rich region (1091 ntp) as well as the genes for the mitochondrial small-subunit rRNA, tRNA(f-met), tRNA(gln), and tRNA(ile), and portions of the ND2 and tRNA(Val) genes. This sequence differs from the corresponding segment of the D. yakuba mtDNA by base substitutions at 0.1% and 0.8% of the positions in the coding and noncoding regions, respectively. The higher divergence due to base substitutions in the A + T-rich region is accompanied by a greater number of insertions/deletions than in the coding regions. From alignment of the D. teissieri A + T-rich sequence with those of D. yakuba and Drosophila virilis, it appears that the 40% of this sequence that lies adjacent to the tRNA(ile) gene has been highly conserved. Divergence between the entire D. teissieri and D. yakuba mtDNA molecules, estimated from the sequences, was 0.3%; this value is close to the value (0.22%) obtained from the restriction analysis, but 10 times lower than the value estimated from published DNA hybridization results.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink