From Mum to Bum: An Observational Study Protocol to Follow Digestion of Human Milk Oligosaccharides and Glycoproteins from Mother to Preterm Infant
| Autor: | Marieke A. C. Hemels, Richard A. van Lingen, Jannie G. E. Henderickx, Esther J d'Haens, Clara Belzer, Mariëtte E. Schoorlemmer, Jan Knol, Astrid Giezen |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
gastric aspirate Neonatal intensive care unit Proteome Oligosaccharides Physiology microbiome Gut flora Feces Clinical Protocols Microbiologie Medicine TX341-641 Infant Nutritional Physiological Phenomena Gastrointestinal tract Nutrition and Dietetics biology Microbiota Human milk human milk Milk Proteins Infant Formula nutrition Research Design Cohort Gestation Digestion Female Infant Premature Microbiology Article Preterm microbiota Humans Microbiome MolEco Glycoproteins VLAG Nutrition Milk Human business.industry Nutrition. Foods and food supply Gastric aspirate Infant Newborn Infant biology.organism_classification infant Gastrointestinal Microbiome Postnatal age feces business preterm Food Science |
| Zdroj: | Nutrients, Vol 13, Iss 3430, p 3430 (2021) Nutrients 13 (2021) 10 Nutrients Volume 13 Issue 10 Nutrients, 13(10) |
| ISSN: | 2072-6643 |
| Popis: | The nutritional requirements of preterm infants are challenging to meet in neonatal care, yet crucial for their growth, development and health. Aberrant maturation of the gastrointestinal tract and the microbiota could affect the digestion of human milk and its nutritional value considerably. Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk. This pilot study will be an observational, single-center study performed at the Neonatal Intensive Care Unit at Isala Women and Children’s Hospital (Zwolle, The Netherlands). A cohort of thirty mother–infant pairs (preterm ≤30 weeks of gestation, n = 15 full-term 37–42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age. We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants. A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements will be performed. The role of the microbiota in infant growth and development is often overlooked yet offers opportunities to advance neonatal care. The ‘From Mum to Bum’ study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated. By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or ‘From Mum to Bum’. |
| Databáze: | OpenAIRE |
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