Placental antiangiogenic prolactin fragments are increased in human and rat maternal diabetes

Autor: Anne Vambergue, Jérôme Delplanque, Amar Abderrahmani, P. Perimenis, L. Devisme, Emmanuelle Moitrot, Thomas Bouckenooghe, Pierre Fontaine, Philippe Froguel, Laurent Storme, Stéphane Lobbens, P. Gosset, B. Duvillie, E. Eury
Rok vydání: 2014
Předmět:
Zdroj: Biochimica et biophysica acta. 1842(9)
ISSN: 0006-3002
Popis: Introduction/objectivesThe role of the placenta in diabetic mothers on fetal development and programming is unknown. Prolactin (PRL) produced by decidual endometrial cells may have an impact. Although full-length PRL is angiogenic, the processed form by bone morphogenetic protein-1 (BMP-1) and/or cathepsin D (CTSD) is antiangiogenic.The objectives were to investigate the involvement of decidual PRL and its antiangiogenic fragments in placentas from type-1 diabetic women (T1D) and from pregnant diabetic rats with lower offspring weights than controls.MethodsPRL, BMP-1, and CTSD gene expressions and PRL protein level were assessed in T1D placentas (n=8) at delivery and compared to controls (n=5). Wistar rats received, at day 7 of pregnancy, streptozotocin (STZ) (n=5) or nicotinamide (NCT) plus STZ (n=9) or vehicle (n=9). Placental whole-genome gene expression and PRL western blots were performed at birth.ResultsIn human placentas, PRL (p
Databáze: OpenAIRE