Placental antiangiogenic prolactin fragments are increased in human and rat maternal diabetes
Autor: | Anne Vambergue, Jérôme Delplanque, Amar Abderrahmani, P. Perimenis, L. Devisme, Emmanuelle Moitrot, Thomas Bouckenooghe, Pierre Fontaine, Philippe Froguel, Laurent Storme, Stéphane Lobbens, P. Gosset, B. Duvillie, E. Eury |
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Rok vydání: | 2014 |
Předmět: |
Adult
medicine.medical_specialty endocrine system endocrine system diseases Offspring Placenta Blotting Western Cathepsin D Real-Time Polymerase Chain Reaction Bone Morphogenetic Protein 1 Diabetes Mellitus Experimental Fetal Development Immunoenzyme Techniques Pregnancy Internal medicine Diabetes mellitus medicine Animals Humans RNA Messenger Rats Wistar Molecular Biology Pancreas Fetus business.industry Reverse Transcriptase Polymerase Chain Reaction Diabetes medicine.disease Streptozotocin Prolactin Rats medicine.anatomical_structure Endocrinology Diabetes Mellitus Type 1 Case-Control Studies embryonic structures Molecular Medicine Female Decidual prolactin-related protein business Vasoinhibin hormones hormone substitutes and hormone antagonists Biomarkers medicine.drug Hormone |
Zdroj: | Biochimica et biophysica acta. 1842(9) |
ISSN: | 0006-3002 |
Popis: | Introduction/objectivesThe role of the placenta in diabetic mothers on fetal development and programming is unknown. Prolactin (PRL) produced by decidual endometrial cells may have an impact. Although full-length PRL is angiogenic, the processed form by bone morphogenetic protein-1 (BMP-1) and/or cathepsin D (CTSD) is antiangiogenic.The objectives were to investigate the involvement of decidual PRL and its antiangiogenic fragments in placentas from type-1 diabetic women (T1D) and from pregnant diabetic rats with lower offspring weights than controls.MethodsPRL, BMP-1, and CTSD gene expressions and PRL protein level were assessed in T1D placentas (n=8) at delivery and compared to controls (n=5). Wistar rats received, at day 7 of pregnancy, streptozotocin (STZ) (n=5) or nicotinamide (NCT) plus STZ (n=9) or vehicle (n=9). Placental whole-genome gene expression and PRL western blots were performed at birth.ResultsIn human placentas, PRL (p |
Databáze: | OpenAIRE |
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