Basonuclin 2 has a function in the multiplication of embryonic craniofacial mesenchymal cells and is orthologous to disco proteins
| Autor: | Fabrice Daubigney, Masatake Araki, Anna Maciejewski-Duval, Ken Ichi Yamamura, Guillaume Soubigou, Cyril Bouche, Amandine Vanhoutteghem, Françoise Hervé, Philippe Djian, Brigitte Delhomme, Kimi Araki |
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| Přispěvatelé: | Régulation de la transcription et maladies génétiques (RTMG), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2009 |
| Předmět: |
Male
Mammalian embryology Biology DNA-binding protein Cell Line Craniofacial Abnormalities 03 medical and health sciences Mice 0302 clinical medicine Tongue Animals Drosophila Proteins [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Messenger Fluorescent Antibody Technique Indirect Peptide sequence Gene 030304 developmental biology Cell Proliferation Oligonucleotide Array Sequence Analysis Genetics Zinc finger Regulation of gene expression Mice Knockout 0303 health sciences Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction Skull Gene Expression Regulation Developmental Mesenchymal Stem Cells Zinc Fingers Biological Sciences Blotting Northern Embryo Mammalian Embryonic stem cell Cleft Palate DNA-Binding Proteins Animals Newborn Female human activities 030217 neurology & neurosurgery Drosophila Protein Transcription Factors |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2009, 106 (34), pp.14432-7. ⟨10.1073/pnas.0905840106⟩ |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0905840106⟩ |
| Popis: | Basonuclin 2 is a recently discovered zinc finger protein of unknown function. Its paralog, basonuclin 1, is associated with the ability of keratinocytes to multiply. The basonuclin zinc fingers are closely related to those of the Drosophila proteins disco and discorelated, but the relation between disco proteins and basonuclins has remained elusive because the function of the disco proteins in larval head development seems to have no relation to that of basonuclin 1 and because the amino acid sequence of disco, apart from the zinc fingers, also has no similarity to that of the basonuclins. We have generated mice lacking basonuclin 2. These mice die within 24 h of birth with a cleft palate and abnormalities of craniofacial bones and tongue. In the embryonic head, expression of the basonuclin 2 gene is restricted to mesenchymal cells in the palate, at the periphery of the tongue, and in the mesenchymal sheaths that surround the brain and the osteocartilagineous structures. In late embryos, the rate of multiplication of these mesenchymal cells is greatly diminished. Therefore, basonuclin 2 is essential for the multiplication of craniofacial mesenchymal cells during embryogenesis. Non- Drosophila insect databases available since 2008 reveal that the basonuclins and the disco proteins share much more extensive sequence and gene structure similarity than noted when only Drosophila sequences were examined. We conclude that basonuclin 2 is both structurally and functionally the vertebrate ortholog of the disco proteins. We also note the possibility that some human craniofacial abnormalities are due to a lack of basonuclin 2. |
| Databáze: | OpenAIRE |
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