The balance of regulatory and stimulatory B cell subsets in breast cancer draining lymph nodes correlates with tumor prognostic factors
| Autor: | Morteza Samadi, Abdolrasoul Talei, Sahar Shariati, Fereshteh Mehdipour, Abbas Ghaderi, Reza Rasolmali |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Axillary lymph nodes Regulatory B cells Programmed Cell Death 1 Receptor B-Lymphocyte Subsets Breast Neoplasms Biology 030226 pharmacology & pharmacy General Biochemistry Genetics and Molecular Biology B7-H1 Antigen 03 medical and health sciences 0302 clinical medicine Breast cancer Immune system medicine Humans General Pharmacology Toxicology and Pharmaceutics B cell Aged B-Lymphocytes Regulatory Apyrase Cancer General Medicine Middle Aged medicine.disease Flow Cytometry Prognosis 030104 developmental biology medicine.anatomical_structure Axilla Cancer research Female Lymph B7-2 Antigen Lymph Nodes CD80 |
| Zdroj: | Life sciences. 257 |
| ISSN: | 1879-0631 |
| Popis: | Aims B cells can promote or inhibit immune responses against breast cancer. We investigated changes in the frequency of B cells with stimulatory or regulatory capacity in breast tumor draining lymph nodes during cancer progression. Main methods We isolated mononuclear cells from fresh axillary lymph nodes (LNs) of 44 patients with breast cancer and stained lymphocytes with antibodies against CD19, CD80, CD86, CD39 and CD73. To assess programmed death-1 (PD-1) and PD-ligand 1 (PD-L1) expression, lymphocytes were briefly stimulated, stained for CD19, PD-1 and PD-L1, and examined with flow cytometry. Key findings The frequency of CD80+ B cells was higher in nonmetastatic lymph nodes, while the percentage of CD86+ B cells showed a positive relationship with higher tumor grade and higher numbers of involved LNs. A small proportion of unstimulated B cells expressed PD-1 or PD-L1 but these molecules were rapidly upregulated on B cells following activation. The frequency of stimulated PD-L1+ B cells showed an inverse association with estrogen and progesterone receptor expression and a nonsignificant positive association with tumor grade. In addition, the percentage of unstimulated PD-1+ B cells was higher in patients with higher-grade tumors. CD73 expression on B cells was associated with lower numbers of involved LNs, and the frequency of CD39+ B cells was higher in patients with larger tumors. Significance CD86+, CD39+, PD-1+ and PD-L1+ B cells showed associations with poor prognostic factors, therefore their potential role in the suppression of the immune responses against breast cancer should be evaluated in greater detail. |
| Databáze: | OpenAIRE |
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