Diarrhea caused by rotavirus A, B, and C in suckling piglets from southern Brazil: molecular detection and histologic and immunohistochemical characterization
| Autor: | Tatiane T. Watanabe, Elis Lorenzetti, Priscila Zlotowski, David Driemeier, Raquel Aparecida Sales da Cruz, Paula Rodrigues de Almeida, Amauri A Alfieri |
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| Rok vydání: | 2018 |
| Předmět: |
Diarrhea
Rotavirus 0301 basic medicine Microbiological culture Swine 040301 veterinary sciences viruses 030106 microbiology medicine.disease_cause Rotavirus Infections Disease Outbreaks 0403 veterinary science Feces 03 medical and health sciences medicine Animals Full Scientific Reports Phylogeny Swine Diseases biology General Veterinary Outbreak Sapovirus 04 agricultural and veterinary sciences biology.organism_classification Immunohistochemistry Virology Animals Suckling Kobuvirus Norovirus medicine.symptom Porcine epidemic diarrhea virus Brazil |
| Zdroj: | Journal of Veterinary Diagnostic Investigation |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/1040638718756050 |
| Popis: | Rotavirus (RV) is an important viral pathogen causing diarrhea in piglets and other mammals worldwide. We describe 34 cases from 4 diarrheal outbreaks caused by RV in unvaccinated farrowing units in southern Brazil from 2011 to 2013. We performed autopsy, histologic examinations, bacterial culture, RV immunohistochemistry (IHC), and enteric virus detection through molecular assays for rotavirus A, B, and C, transmissible gastroenteritis virus, porcine epidemic diarrhea virus, sapovirus, norovirus, and kobuvirus. Histologically, villus atrophy (29 of 34) and epithelial vacuolation (27 of 34) occurred in all 4 outbreaks. Cell debris in the lamina propria occurred in 20 cases, mostly from outbreaks A (8 of 11), C (4 of 6), and D (7 of 11). IHC was positive for RV in 21 of 34 samples. RT-PCR was positive for RV in 20 of 30 samples; RV-C was the most frequently detected RV ( n = 17). Kobuvirus was detected in 11 samples, and, in 3 of them, there was single detection of this enteric virus. |
| Databáze: | OpenAIRE |
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