Pollen Cryopreservation in Jackfruit for Crop Improvement (Poster)
| Autor: | Navya B. L., Rajasekharan P. E., Sridhar Gutam |
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| Rok vydání: | 2020 |
| Předmět: | |
| DOI: | 10.5281/zenodo.7973761 |
| Popis: | Jackfruit is an important edible fruit bearing tree of India. The fruits have multifarious uses, high nutritive value and market potential. The trees exhibit wide range of diversity with respect to fruit character and bulb quality. There is lack of potential varieties with high quality fruits. Pollen is an important entity in transfer of genetic traits. Pollen mediated trait transfer can be effectively utilized in the crop improvement programmes. The jackfruit cultivars show asynchronous flowering pattern which is a barrier in breeding program. Hence an effective and long-term storage of pollens can be successfully achieved through cryopreservation. The male spikes were collected during anthesis at 2-3 pm, pollen grains were extracted and dried in zeolite desiccator for an hour, The pre-storage viability assessment was carried out by hanging drop technique using Brewbaker and Kwack (BK) medium containing 5% sucrose. Then the pollen were packed with butter paper enclosed in aluminium pouch and were rapidly plunged into the cryobiological system containing liquid nitrogen. The post storage germination assessment was done in similar way. The scanning electron microscopy (SEM) was carried out, length and wide of the pollen was recorded. The results indicated that germination percentage of fresh pollen was 72% and was 69% after three weeks of cryopreservation. The illustrates that there I no decline in the viability of pollens after cryopreservation. Therefore, it can be concluded that jackfruit pollens can be cryopreserved successfully and can be used in breeding programmes for transfer of quality traits and to broaden the genetic base by excluding the limitation if time and season. Poster No.. 70 |
| Databáze: | OpenAIRE |
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