Physiological differences and changes in global DNA methylation levels in Agave angustifolia Haw. albino variant somaclones during the micropropagation process
Autor: | Felipe Barredo Pool, Francisco Espadas, Fátima Duarte-Aké, Manuel L. Robert, Jorge M. Santamaría, Eduardo Castillo-Castro, Clelia De-la-Peña |
---|---|
Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine Chlorophyll Photoperiod Malates Plant Science 01 natural sciences Tissue Culture Techniques 03 medical and health sciences Agave Chromosome Segregation Botany Epigenetics Gene Genetics biology General Medicine DNA Methylation biology.organism_classification Phenotype Carotenoids Chromatin Clone Cells 030104 developmental biology Micropropagation Shoot DNA methylation Plant Stomata Agronomy and Crop Science 010606 plant biology & botany |
Zdroj: | Plant cell reports. 35(12) |
ISSN: | 1432-203X |
Popis: | Global DNA methylation changes caused by in vitro conditions are associated with the subculturing and phenotypic variation in Agave angustifolia Haw. While the relationship between the development of albinism and in vitro culture is well documented, the role of epigenetic processes in this development leaves some important questions unanswered. During the micropropagation of Agave angustifolia Haw., we found three different phenotypes, green (G), variegated (V) and albino (A). To understand the physiological and epigenetic differences among the somaclones, we analyzed several morphophysiological parameters and changes in the DNA methylation patterns in the three phenotypes during their in vitro development. We found that under in vitro conditions, the V plantlets maintained their CAM photosynthetic capacity, while the A variant showed no pigments and lost its CAM photosynthetic ability. Epigenetic analysis revealed that global DNA methylation increased in the G phenotype during the first two subcultures. However, after that time, DNA methylation levels declined. This hypomethylation correlated with the appearance of V shoots in the G plantlets. A similar correlation occurred in the V phenotype, where an increase of 2 % in the global DNA methylation levels was correlated with the generation of A shoots in the V plantlets. This suggests that an “epigenetic stress memory” during in vitro conditions causes a chromatin shift that favors the generation of variegated and albino shoots. |
Databáze: | OpenAIRE |
Externí odkaz: |