A German external quality survey of diagnostic microbiology of respiratory tract infections in patients with cystic fibrosis
| Autor: | Jürgen Heesemann, Beate Balke, Michael Hogardt, Sebastian Suerbaum, Susanne Häuβler, Sabine Schmoldt |
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| Přispěvatelé: | Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hanover, Germany. |
| Rok vydání: | 2008 |
| Předmět: |
Quality Control
Pulmonary and Respiratory Medicine Quality Assurance Health Care Burkholderia cenocepacia Microbial Sensitivity Tests medicine.disease_cause Cystic fibrosis Microbiology External quality control programme Germany External quality assessment Humans Medicine Infection control Pseudomonas Infections Pediatrics Perinatology and Child Health Respiratory Tract Infections Clinical microbiology laboratories Bacteriological Techniques biology Respiratory tract infections business.industry Pseudomonas aeruginosa Burkholderia multivorans Burkholderia Infections Staphylococcal Infections biology.organism_classification medicine.disease Staphylococcus aureus Pediatrics Perinatology and Child Health business |
| Zdroj: | Journal of Cystic Fibrosis. 7:7-14 |
| ISSN: | 1569-1993 |
| Popis: | Background The goal of this pilot study was to design an external quality assessment (EQA) scheme for German cystic fibrosis (CF) clinical microbiology laboratories. Therefore, a multicentre study of 18 German CF laboratories was performed to evaluate their proficiency in analyzing CF respiratory secretions. Methods Simulated clinical specimens containing a set of four frequent CF pathogens, namely two Pseudomonas aeruginosa strains differing in morphotype ( mucoid versus non-mucoid ) and resistotype, one Staphylococcus aureus strain and one Burkholderia multivorans strain, were distributed to each laboratory. Isolation, identification and antimicrobial susceptibility testing (AST) of any bacterial pathogen present and completion of a questionnaire about applied microbiological protocols were requested. Results Three of four strains were isolated and identified correctly by almost all laboratories. B. multivorans was once misidentified as Burkholderia cenocepacia . Fourteen laboratories failed to detect the second multidrug resistant P. aeruginosa isolate. AST errors occurred most often for P. aeruginosa 2 followed by B. multivorans, P. aeruginosa 1 and S. aureus . Evaluation of the questionnaires revealed major differences in cultivation and identification techniques applied by the participating laboratories. Conclusions A periodical EQA programme for German CF laboratories and standardized microbiological procedures seem to be necessary to advance diagnostic microbiology employed on CF respiratory tract specimens and may help to improve anti-infective treatment and infection control practices for CF patients. |
| Databáze: | OpenAIRE |
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