Effect of melatonin on motor performance and brain cortex mitochondrial function during ethanol hangover

Autor: Analía Czerniczyniec, Rodolfo A. Cutrera, Silvia Lores-Arnaiz, Analía G. Karadayian, Juanita Bustamante
Rok vydání: 2014
Předmět:
Male
MITOCHONDRIAL MEMBRANE POTENTIAL
MELATONIN
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
Antioxidant
medicine.medical_treatment
ETHANOL HANGOVER
Nitric Oxide Synthase Type II
Ciencias de la Salud
Nitric Oxide Synthase Type I
Motor Activity
medicine.disease_cause
Antioxidants
Melatonin
Mice
chemistry.chemical_compound
Internal medicine
medicine
Animals
OXIDATIVE STRESS
Respiratory system
Cerebral Cortex
Membrane Potential
Mitochondrial
HEPES
Ethanol
Chemistry
General Neuroscience
Central Nervous System Depressants
Hydrogen Peroxide
Metabolism
Mitochondria
MITOCHONDRIAL FUNCTION
Oxygen
Otras Ciencias de la Salud
EGTA
Endocrinology
Biochemistry
MOTOR COORDINATION
Nitrogen Oxides
Alcohol-Related Disorders
hormones
hormone substitutes
and hormone antagonists
Oxidative stress
medicine.drug
Zdroj: Neuroscience. 269:281-289
ISSN: 0306-4522
DOI: 10.1016/j.neuroscience.2014.03.062
Popis: Increased reactive oxygen species generation and mitochondrial dysfunction occur during ethanol hangover. The aim of this work was to study the effect of melatonin pretreatment on motor performance and mitochondrial function during ethanol hangover. Male mice received melatonin solution or its vehicle in drinking water during 7 days and i.p. injection with EtOH (3.8 g/kg BW) or saline at the eighth day. Motor performance and mitochondrial function were evaluated at the onset of hangover (6 h after injection). Melatonin improved motor coordination in ethanol hangover mice. Malate–glutamate-dependent oxygen uptake was decreased by ethanol hangover treatment and partially prevented by melatonin pretreatment. Melatonin alone induced a decrease of 30% in state 4 succinate-dependent respiratory rate. Also, the activity of the respiratory complexes was decreased in melatonin-pretreated ethanol hangover group. Melatonin pretreatment before the hangover prevented mitochondrial membrane potential collapse and induced a 79% decrement of hydrogen peroxide production as compared with ethanol hangover group. Ethanol hangover induced a 25% decrease in NO production. Melatonin alone and as a pretreatment before ethanol hangover significantly increased NO production by nNOS and iNOS as compared with control groups. No differences were observed in nNOS protein expression, while iNOS expression was increased in the melatonin group. Increased NO production by melatonin could be involved in the decrease of succinate-dependent oxygen consumption and the inhibition of complex IV observed in our study. Melatonin seems to act as an antioxidant agent in the ethanol hangover condition but also exhibited some dual effects related to NO metabolism. Fil: Karadayian, Analia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Bustamante, J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Czerniczyniec, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Cutrera, Rodolfo Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Lores Arnaiz, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Databáze: OpenAIRE
Externí odkaz: