Palmitate uptake by hepatocyte monolayers. Effect of albumin binding
| Autor: | A B Fleischer, B A Luxon, E L Forker, W O Shurmantine |
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| Rok vydání: | 1986 |
| Předmět: |
Time Factors
Cell Palmitic Acid Palmitic Acids Endocytosis Dissociation (chemistry) Palmitic acid chemistry.chemical_compound Albumins medicine Animals Cells Cultured chemistry.chemical_classification biology Albumin Fatty acid Biological Transport General Medicine Rats medicine.anatomical_structure Liver chemistry Biochemistry Hepatocyte biology.protein Adsorption Collagen Research Article Organic anion |
| Zdroj: | Journal of Clinical Investigation. 77:964-970 |
| ISSN: | 0021-9738 |
| DOI: | 10.1172/jci112397 |
| Popis: | The uptake of 14C-palmitate by rat liver cell monolayers is depressed by binding of the fatty acid to albumin. When the uptake flux is divided by the concentration of free palmitate in the bathing medium, however, the resulting clearance is approximately 14 times greater in the presence of albumin than in its absence. These findings are not accounted for by the different diffusion rates of free and bound palmitate across an unstirred fluid layer, nor attributable to nonequilibrium binding. Instead we argue that the most plausible explanation is accelerated dissociation of albumin-palmitate complexes mediated by the cell surface--an interpretation that also explains the uptake kinetics of other albumin-bound organic anions by perfused rat liver. |
| Databáze: | OpenAIRE |
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