Regulation of Bed Nucleus of the Stria Terminalis PACAP Expression by Stress and Corticosterone
| Autor: | Sayamwong E. Hammack, Victor May, Kimberly R. Lezak, Jay Schulkin, Karen M. Braas, Carolyn W. Roman, William A. Falls, Kristin C. Schutz |
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| Rok vydání: | 2014 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Article Rats Sprague-Dawley Cellular and Molecular Neuroscience chemistry.chemical_compound Corticosterone Internal medicine medicine Animals Neurochemistry RNA Messenger Gene Stressor Septal nuclei General Medicine Rats Stria terminalis medicine.anatomical_structure Endocrinology Real-time polymerase chain reaction chemistry Pituitary Adenylate Cyclase-Activating Polypeptide Septal Nuclei Psychology Nucleus Stress Psychological hormones hormone substitutes and hormone antagonists Receptors Pituitary Adenylate Cyclase-Activating Polypeptide Type I |
| Zdroj: | Journal of Molecular Neuroscience. 54:477-484 |
| ISSN: | 1559-1166 0895-8696 |
| DOI: | 10.1007/s12031-014-0269-8 |
| Popis: | Single-nucleotide polymorphisms (SNPs) in the genes for pituitary adenylyl cyclase-activating peptide (PACAP) and the PAC1 receptor have been associated with stress-related psychiatric disorders. Although, from recent work, we have argued that stress-induced PACAP expression in the bed nucleus of the stria terminalis (BNST) may mediate stress-related psychopathology, it is unclear whether stress-induced increases in BNST PACAP expression require acute or repeated stressor exposure and whether increased BNST PACAP expression is related to stress-induced increases in circulating glucocorticoids. In the current work, we have used real-time quantitative polymerase chain reaction (qPCR) to assess transcript expression in brain punches from rats after stressor exposure paradigms or corticosterone injection. BNST PACAP and PAC1 receptor transcript expression was increased only after 7 days of repeated stressor exposure; no changes in transcript levels were observed 2 or 24 hours after a single-restraint session. Moreover, repeated corticosterone treatment for 7 days was not sufficient to reliably increase BNST PACAP transcript levels, suggesting that stress-induced elevations in corticosterone may not be the primary drivers of BNST PACAP expression. These results may help clarify the mechanisms and temporal processes that underlie BNST PACAP induction for intervention in stress-related anxiety disorders. |
| Databáze: | OpenAIRE |
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