Comparative transcriptomic adaptations of Staphylococcus aureus to the wound environment in non‐diabetic and diabetic mice

Autor: David Dolivo, Steven Lanier, Kai Leung, Thomas Mustoe, Seok Jong Hong, Robert Galiano
Rok vydání: 2022
Předmět:
Zdroj: Wound Repair and Regeneration. 30:541-545
ISSN: 1524-475X
1067-1927
DOI: 10.1111/wrr.13040
Popis: Infection is a major source of complications in delayed diabetic wound healing. Increased understanding of differential bacterial responses to diabetic wounds will enable us to better understand chronic wound pathogenesis. Here we create delayed-healing wounds infected with Staphylococcus aureus in non-diabetic and diabetic mice and used RNA-seq to compare bacterial gene expression profiles 3 or 7 days after infection. Analysis at day 3 demonstrated substantial transcriptomic differences between bacteria colonising non-diabetic and diabetic wound beds. Most of these transcriptional differences resolved by day 7, suggesting normalisation of many bacterial phenotypes later in the diabetic wound healing process. Lingering differentially expressed genes at day 7 were enriched for genes related to carbohydrate metabolism, which includes genes of the lac operon, and capsular polysaccharide synthesis, which includes the cap8 locus. These data encourage further research into host-pathogen interactions in wound healing and how they influence differential outcomes in the diabetic wound environment.
Databáze: OpenAIRE
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