Cell-Peptide Specific Interaction Can Inhibit Mycobacterium tuberculosis H37Rv Infection
| Autor: | Manuel E. Patarroyo, Deisy Carolina Rodriguez, Gabriela Arévalo-Pinzón, Manuel A. Patarroyo, Marina Muñoz, Marisol Ocampo, César Reyes |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Signal peptide
Secondary Transcription Genetic Cell Peptide Plasma protein binding Biochemistry Epitope Epithelium cell Models Cell differentiation Pathology Enzyme activity Peptide sequence Antiinfective agent chemistry.chemical_classification Tumor Genetic transcription Bacterial Polymerase chain reaction Host pathogen interaction Protein secondary structure Human Bacterial Outer Membrane Proteins Protein Structure U937 cell line Immunology Molecular Sequence Data Circular dichroism Microbiology Article Rv3705c protein Mycobacterium tuberculosis Binding site HIGH ACTIVITY BINDING PEPTIDE (HABP) 03 medical and health sciences Genetic Genetics Tuberculosis Humans Amino Acid Sequence Immune response INHIBITION ASSAY Molecular Biology Macrophages Molecular Epithelial Cells Tumor cell line 030104 developmental biology chemistry Myristylation Peptides Models Molecular 0301 basic medicine Glycosylation Unclassified drug Macrophage Gene Expression Protein Structure Secondary Western blotting Synthesis A549 cell line Molecular genetics Priority journal biology Protein interaction BIOINFORMATICS Cell Differentiation Anti-Bacterial Agents Chemistry medicine.anatomical_structure Host-Pathogen Interactions RECEPTOR-LIGAND ASSAY Transcription Protein Binding Immunoblotting Bacterial antigen Molecular model Cell Line Amino acid sequence Immune system Protein Data Bank Cell Line Tumor medicine Electron microscopy Antigens Antigens Bacterial Drug effects Binding Sites Protein localization In vitro study Cell Biology biology.organism_classification Nonhuman Molecular biology Synthetic peptide Cell culture Outer membrane protein Prediction Cytology Controlled study |
| Zdroj: | Repositorio EdocUR-U. Rosario Universidad del Rosario instacron:Universidad del Rosario |
| Popis: | Studying proteins from the M. tuberculosis H37Rv envelop is important for understanding host-pathogen interaction regarding bacterial infection and survival within a host; such knowledge is indispensable regarding studies aimed at developing drugs or vaccines against tuberculosis, a disease which continues to cause more than one million deaths worldwide every year. The present work presents a study of the Rv3705c protein which has been described as being an outer protein. Several servers and bioinformatics' tools were used for predicting its location on mycobacterial surface and a 3D model of the protein was obtained which was then compared to experimental circular dichroism results for its peptides. PCR assays were used for corroborating rv3705c gene presence and transcription in a laboratory strain and immunoblotting and electron microscopy were used for confirming protein localisation on cell envelop. Receptor-ligand assays revealed two peptides having high specific binding (HABPs); peptide 38485 (121DRAFHRVVDRTVGTSGQTTA140) bound to both cell lines used as infection target (U937 and A549 epithelial cell line-derived macrophages) and 38488 (181RLRENVLLQAKVTQSGNAGP200) bound to U937 cells. It was found that peptide 38485 provided significant inhibition regarding mycobacterial entry to both cell lines in in vitro assays. These results led to proposing peptide 38485 as one of the epitopes to be used in future studies aimed at characterising the immune response of functionally important synthetic peptides which could be included in developing a synthetic anti-tuberculosis vaccine. © 2015 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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