Automated Separation of Visceral and Subcutaneous Adiposity in In Vivo Microcomputed Tomographies of Mice
| Autor: | Stefan Judex, Svetlana Lublinsky, Clinton T. Rubin, Yen K. Luu |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Pathology
medicine.medical_specialty X-ray microtomography Intra-Abdominal Fat Normal Distribution Subcutaneous Fat Adipose tissue Biology Sensitivity and Specificity Article Mice In vivo medicine Canny edge detector Animals Radiology Nuclear Medicine and imaging Contouring Radiological and Ultrasound Technology Reproducibility of Results X-Ray Microtomography Microcomputed tomography Computer Science Applications Mice Inbred C57BL Automated algorithm Algorithms Biomedical engineering |
| Zdroj: | Journal of Digital Imaging. 22:222-231 |
| ISSN: | 1618-727X 0897-1889 |
| DOI: | 10.1007/s10278-008-9152-x |
| Popis: | Reflecting its high resolution and contrast capabilities, microcomputed tomography (μCT) can provide an in vivo assessment of adiposity with excellent spatial specificity in the mouse. Herein, an automated algorithm that separates the total abdominal adiposity into visceral and subcutaneous compartments is detailed. This algorithm relies on Canny edge detection and mathematical morphological operations to automate the manual contouring process that is otherwise required to spatially delineate the different adipose deposits. The algorithm was tested and verified with μCT scans from 74 C57BL/6J mice that had a broad range of body weights and adiposity. Despite the heterogeneity within this sample of mice, the algorithm demonstrated a high degree of stability and robustness that did not necessitate changing of any of the initially set input variables. Comparisons of data between the automated and manual methods were in complete agreement (R 2 = 0.99). Compared to manual contouring, the increase in precision and accuracy, while decreasing processing time by at least an order of magnitude, suggests that this algorithm can be used effectively to separately assess the development of total, visceral, and subcutaneous adiposity. As an application of this method, preliminary data from adult mice suggest that a relative increase in either subcutaneous, visceral, or total fat negatively influences skeletal quantity and that fat infiltration in the liver is greatly increased by a high-fat diet. |
| Databáze: | OpenAIRE |
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