Reproduction of bacteriophage T6 and T7 in auxotrophic mutants of E. coli B
| Autor: | Ebels I, Dolman H, Winkler Kc |
|---|---|
| Rok vydání: | 1955 |
| Předmět: |
chemistry.chemical_classification
Methionine biology Auxotrophy General Medicine biology.organism_classification medicine.disease_cause Microbiology Amino acid Bacteriophage Serine chemistry.chemical_compound Biochemistry chemistry medicine Escherichia coli Bacteriophages Tyrosine Leucine Amino Acids Molecular Biology |
| Zdroj: | Antonie van Leeuwenhoek. 21(4) |
| ISSN: | 0003-6072 |
| Popis: | Phage T6 and T7 did not reproduce in mutants ofE. coli auxotrophic for the amino acids methionine, leucine, serine, proline, histidine, tyrosine, phenylalanine and tryptophane unless these amino acids were present in the medium. Very small amounts of these auxotrophic factors (0.01 γ/ml) are able to support phage multiplication. Much care has to be taken to avoid impurities in the medium and to deplete the endogenous reserves of the host cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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