A study of mechanisms involved in vasodilatation induced by resveratrol in isolated porcine coronary artery
| Autor: | Hong-Fang, Li, Zhi-Feng, Tian, Xiao-Qing, Qiu, Jin-Xia, Wu, Peng, Zhang, Zhong-Jian, Jia |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Serotonin Dose-Response Relationship Drug Swine Physiology Vasodilator Agents General Medicine In Vitro Techniques Nitric Oxide Coronary Vessels Nitroarginine Potassium Chloride Vasodilation Serotonin Agents Resveratrol Coronary Circulation Receptors Adrenergic beta Stilbenes Prostaglandins Animals Calcium Female Endothelium Vascular Enzyme Inhibitors |
| Zdroj: | Physiological Research. :365-372 |
| ISSN: | 1802-9973 0862-8408 |
| DOI: | 10.33549/physiolres.930826 |
| Popis: | The present study was designed to investigate the acute relaxing effect of phytoestrogen resveratrol on isolated porcine coronary arteries and to determine the mechanisms underlying its vasodilatation. Rings of porcine coronary arteries were suspended in organ baths containing Krebs-Henseleit solution, and then isometric tension was measured. Resveratrol concentration-dependently relaxed arterial rings precontracted with 30 mM KCl. The IC(50) value of resveratrol was 38.67+/-3.21 microM. Incubation with N(omega)-L-nitro-arginine (L-NNA), endothelium removal or the presence of a potent inhibitor of protein tyrosine phosphatase sodium orthovanadate partly decreased the relaxation induced by resveratrol. However, the relaxation induced by resveratrol was unaffected by the estrogen receptor antagonist tamoxifen, the inhibitor of prostanoid synthesis indomethacin, the antagonist of beta-adrenoceptors propranolol or the protein synthesis inhibitor, cycloheximide. In addition, resveratrol significantly decreased the contractile responses of 5-HT, KCl and CaCl(2), and shifted their cumulative concentration-response curves to the right. These results suggest that the mechanisms of vasorelaxation induced by resveratrol are heterogeneous, two mechanisms participating partially in the relaxation of porcine coronary artery were detected in the study, one being the nitric oxide released from the endothelium, the other causing inhibition of Ca(2+) influx, but estrogen receptors were not involved in resveratrol-induced relaxation. |
| Databáze: | OpenAIRE |
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