Reticuloendotheliosis virus and avian leukosis virus subgroup J synergistically increase the accumulation of exosomal miRNAs

Autor: Venugopal Nair, Defang Zhou, Yongxiu Yao, Jing Zhou, Xusheng Du, Libo Huang, Jingwen Xue, Shuhai He, Ziqiang Cheng, Chengui Li
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Retrovirology, Vol 15, Iss 1, Pp 1-11 (2018)
Retrovirology
ISSN: 1742-4690
DOI: 10.1186/s12977-018-0427-0
Popis: Background Co-infection with avian leukosis virus subgroup J and reticuloendotheliosis virus induces synergistic pathogenic effects and increases mortality. However, the role of exosomal miRNAs in the molecular mechanism of the synergistic infection of the two viruses remains unknown. Results In this study, exosomal RNAs from CEF cells infected with ALV-J, REV or both at the optimal synergistic infection time were analysed by Illumina RNA deep sequencing. A total of 54 (23 upregulated and 31 downregulated) and 16 (7 upregulated and 9 downregulated) miRNAs were identified by comparing co-infection with two viruses, single-infected ALV-J and REV, respectively. Moreover, five key miRNAs, including miR-184-3p, miR-146a-3p, miR-146a-5p, miR-3538 and miR-155, were validated in both exosomes and CEF cells by qRT-PCR. GO annotation and KEGG pathway analysis of the miRNA target genes showed that the five differentially expressed miRNAs participated in virus-vector interaction, oxidative phosphorylation, energy metabolism and cell growth. Conclusions We demonstrated that REV and ALV-J synergistically increased the accumulation of exosomal miRNAs, which sheds light on the synergistic molecular mechanism of ALV-J and REV. Electronic supplementary material The online version of this article (10.1186/s12977-018-0427-0) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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