Taxonomically Different Co-Microsymbionts of a Relict Legume, Oxytropis popoviana, Have Complementary Sets of Symbiotic Genes and Together Increase the Efficiency of Plant Nodulation
| Autor: | Vera I. Safronova, Andrey A. Belimov, Irina G. Kuznetsova, Igor A. Tikhonovich, Jan V. Puhalsky, Elizaveta R. Chirak, Evgeny E. Andronov, Alla V. Verkhozina, Anna L. Sazanova |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Root nodule Physiology 030106 microbiology Plant Root Nodulation Oxytropis 03 medical and health sciences Gene Expression Regulation Plant Botany Bradyrhizobium Internal transcribed spacer Symbiosis Gene Legume Phylogeny biology Mesorhizobium food and beverages General Medicine Gene Expression Regulation Bacterial biology.organism_classification 030104 developmental biology Identification (biology) Agronomy and Crop Science Gene Deletion |
| Zdroj: | Molecular plant-microbe interactions : MPMI. 31(8) |
| ISSN: | 0894-0282 |
| Popis: | Ten rhizobial strains were isolated from root nodules of a relict legume Oxytropis popoviana Peschkova. For identification of the isolates, sequencing of rrs, the internal transcribed spacer region, and housekeeping genes recA, glnII, and rpoB was used. Nine fast-growing isolates were Mesorhizobium-related; eight strains were identified as M. japonicum and one isolate belonged to M. kowhaii. The only slow-growing isolate was identified as a Bradyrhizobium sp. Two strains, M. japonicum Opo-242 and Bradyrhizobium sp. strain Opo-243, were isolated from the same nodule. Symbiotic genes of these isolates were searched throughout the whole-genome sequences. The common nodABC genes and other symbiotic genes required for plant nodulation and nitrogen fixation were present in the isolate Opo-242. Strain Opo-243 did not contain the principal nod, nif, and fix genes; however, five genes (nodP, nodQ, nifL, nolK, and noeL) affecting the specificity of plant-rhizobia interactions but absent in isolate Opo-242 were detected. Strain Opo-243 could not induce nodules but significantly accelerated the root nodule formation after coinoculation with isolate Opo-242. Thus, we demonstrated that taxonomically different strains of the archaic symbiotic system can be co-microsymbionts infecting the same nodule and promoting the nodulation process due to complementary sets of symbiotic genes. |
| Databáze: | OpenAIRE |
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