An RNA Origami Octahedron with Intrinsic siRNAs for Potent Gene Knockdown
| Autor: | Veronica Liv Andersen, Hans Christian Høiberg, Ebbe Sloth Andersen, Steffen M. Sparvath, Jørgen Kjems |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Small interfering RNA 01 natural sciences Applied Microbiology and Biotechnology chemistry.chemical_compound RNA interference 010608 biotechnology Gene silencing Animals Humans Nanotechnology RNA Small Interfering Gene knockdown biology Chemistry 010401 analytical chemistry RNA General Medicine 0104 chemical sciences Cell biology Nanostructures Gene Knockdown Techniques Nucleic acid biology.protein Molecular Medicine Nucleic Acid Conformation RNA Interference DNA Dicer |
| Zdroj: | Høiberg, H C, Sparvath, S M, Andersen, V L, Kjems, J & Andersen, E S 2019, ' An RNA origami octahedron with intrinsic siRNAs for potent gene knockdown ', Biotechnology Journal, vol. 14, no. 1, 1700634 . https://doi.org/10.1002/biot.201700634 |
| ISSN: | 1860-7314 |
| Popis: | The fields of DNA and RNA nanotechnology have established nucleic acids as valuable building blocks for functional nanodevices with applications in nanomedicine. Here, a simple method for designing and assembling a three‐dimensional (3D) scaffolded RNA origami wireframe structure with intrinsic functioning small interfering RNAs (siRNAs) embedded is introduced. Uniquely, the method uses an mRNA fragment as scaffold strand, which is folded by sequence‐complementarity of nine shorter synthetic strands. High‐yield production of the intended 3D structure is verified by transmission electron microscopy (TEM). Production of functional siRNAs is facilitated by incorporating recognition sites for Dicer at selected locations in the structure and efficient silencing of a target reporter gene is demonstrated. |
| Databáze: | OpenAIRE |
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