Alterations in Bone and Erythropoiesis in Hemolytic Anemia: Comparative Study in Bled, Phenylhydrazine-Treated and Plasmodium-Infected Mice
| Autor: | Tatiana Scorza, David Scott Bohle, Jaime Sanchez Dardon, Esther Dalko, Mathieu Dumais, Corine Martineau, Véronique Gaudreault, Diane Tshikudi Malu, Olha Kevorkova, Erin L. Dodd, Robert Moreau, Hugo Romero |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Hemolytic anemia
Plasmodium Mouse Reticulocytosis Osteoporosis Red Cells Osteopenia and Osteoporosis lcsh:Medicine Osteoclasts Bone remodeling Mice Bone Marrow Molecular Cell Biology Erythropoiesis lcsh:Science Cellular Stress Responses Mice Inbred BALB C Multidisciplinary Anemia Animal Models Hematology Phenylhydrazines medicine.anatomical_structure Infectious Diseases Medicine Female medicine.symptom medicine.drug Research Article medicine.medical_specialty Anemia Hemolytic Biology Bone and Bones Model Organisms Erythroid Cells Internal medicine medicine Parasitic Diseases Animals lcsh:R Hemolytic Anemia medicine.disease Bone Marrow Failure Hematopoiesis Malaria Hemoglobinopathies Endocrinology Erythropoietin Immunology Women's Health lcsh:Q Bone marrow Reactive Oxygen Species |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 9, p e46101 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Sustained erythropoiesis and concurrent bone marrow hyperplasia are proposed to be responsible for low bone mass density (BMD) in chronic hemolytic pathologies. As impaired erythropoiesis is also frequent in these conditions, we hypothesized that free heme may alter marrow and bone physiology in these disorders. Bone status and bone marrow erythropoiesis were studied in mice with hemolytic anemia (HA) induced by phenylhydrazine (PHZ) or Plasmodium infection and in bled mice. All treatments resulted in lower hemoglobin concentrations, enhanced erythropoiesis in the spleen and reticulocytosis. The anemia was severe in mice with acute hemolysis, which also had elevated levels of free heme and ROS. No major changes in cellularity and erythroid cell numbers occurred in the bone marrow of bled mice, which generated higher numbers of erythroid blast forming units (BFU-E) in response to erythropoietin. In contrast, low numbers of bone marrow erythroid precursors and BFU-E and low concentrations of bone remodelling markers were measured in mice with HA, which also had blunted osteoclastogenesis, in opposition to its enhancement in bled mice. The alterations in bone metabolism were accompanied by reduced trabecular bone volume, enhanced trabecular spacing and lower trabecular numbers in mice with HA. Taken together our data suggests that hemolysis exerts distinct effects to bleeding in the marrow and bone and may contribute to osteoporosis through a mechanism independent of the erythropoietic stress. |
| Databáze: | OpenAIRE |
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