Changes in immunological recovery in patients who received post-transplant G-CSF or GM-CSF after autologous peripheral blood stem cell transplantation (PBSCT)
| Autor: | T Dogru, Ahmet Özet, Fikret Arpaci, Mustafa Turan, M I Yilmaz, Murat Beyzadeoglu, A Sengul, Atilla Yalçin, Şeref Kömürcü, Bekir Öztürk |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male CD34 Granulocyte Transplantation Autologous CD19 Immune system Antigen Antigens CD Granulocyte Colony-Stimulating Factor Humans Medicine Lymphocyte Count Peripheral Blood Stem Cell Transplantation biology business.industry Graft Survival Granulocyte-Macrophage Colony-Stimulating Factor Hematology Immunoglobulin Isotypes Transplantation Kinetics Haematopoiesis medicine.anatomical_structure Immune System Immunology biology.protein Female business CD8 |
| Zdroj: | Haematologia. 32:253-264 |
| ISSN: | 1568-5594 0017-6559 |
| DOI: | 10.1163/15685590260461066 |
| Popis: | In this prospective study, the effects of granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) on immunological reconstitution after autologous peripheral blood stem cell transplantation (PBSCT) were investigated for 6 months. Thirty-five patients received G-CSF 5 microg/kg per day and 26 patients received GM-CSF SC 5 microg/kg per day from day 1 to leukocyte engraftment (>1000 per mm3). Peripheral blood samples were obtained on 14, 28, 100, and 180 days after transplantation for immunological evaluation. CD3+, CD4+, CD8+, CD19+, and CD56+ cells were analysed by flow cytometry. Immunoglobulin levels (IgG, IgA, and IgM) and complement levels (C3c and C4) were measured by nephelometry. Both G-CSF and GM-CSF groups were comparable with respect to age, sex, the period from diagnosis to transplantation, total nucleated cells infused, the number of CD34+ cells, conditioning regimens (TBI and non-TBI), and post-transplant infection. CD3+ and CD8+ cells on day 14 following autologous PBSCT + G-CSF were significantly higher than following autologous PBSCT + GM-CSF (p = 0.008 and p = 0.021, respectively). The number of CD4 cells and the CD4/CD8 ratio were not different at several time points between the two groups. CD19+, CD56+ cells and immunoglobulin levels showed a faster recovery pattern in the autologous PBSCT + G-CSF group. The effect of G-CSF on immune reconstitution after autologous PBSCT is more prominent than that of GM-CSF. The possible role of haematopoietic growth factor on immune recovery and its clinical importance should be investigated in further studies. |
| Databáze: | OpenAIRE |
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