Type I insulin-like growth factor receptor expression in different developmental stages of human thymocytes
| Autor: | R. Kooijman, Ben J.M. Zegers, L. E. Scholtens, G T Rijkers |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
CD4-Positive T-Lymphocytes
medicine.medical_specialty T-Lymphocytes Endocrinology Diabetes and Metabolism T cell medicine.medical_treatment Fluorescent Antibody Technique Thymus Gland CD8-Positive T-Lymphocytes Insulin-Like Growth Factor Receptor Biology Binding Competitive Receptor IGF Type 1 Radioligand Assay Endocrinology Insulin-Like Growth Factor II Internal medicine medicine Humans Insulin Insulin-Like Growth Factor I Receptor Cells Cultured DNA synthesis Growth factor DNA T lymphocyte Flow Cytometry Thymocyte medicine.anatomical_structure CD8 |
| Zdroj: | Journal of Endocrinology. 147:203-209 |
| ISSN: | 1479-6805 0022-0795 |
| Popis: | Insulin-like growth factor-I (IGF-I) has been implicated in playing a regulatory role in T cell development and in T cell function. We demonstrate the presence of type I IGF receptors on human thymocytes using radioligand binding assays and flowcytometric analysis. The relative potencies of IGF-I, IGF-II and insulin for competition with 125I-IGF-I indicate the presence of type I IGF receptors. Scatchard analysis revealed that the average number of receptors per thymocyte is 257 ± 28 with a Kd of 0·12 ±0·01 With multicolour flowcytometry using a type I IGF receptor specific monoclonal antibody (αIR3), we show that CD4− CD8− cells express 3-4 times more receptors per cell as compared with CD4+ CD8+, CD4+ CD8− and CD4− CD8+ cells. IGF-I directly stimulated DNA synthesis of thymocytes and potentiated DNA synthesis in mitogen-activated thymocytes. These results indicate that IGF-I can influence T cell development in humans at the level of the thymus. Journal of Endocrinology (1995) 147, 203–209 |
| Databáze: | OpenAIRE |
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