Preferential binding of E. coli with type 1 fimbria to d- mannobiose with the Manα1→2Man structure
| Autor: | Katsumi Ajisaka, Ryo Kuji, Kiyoshi Furukawa, Tatsuo Miyazaki, Nozomi Ishii, Kaoru Yuki, Ichiro Matsuo, Kaori Sato |
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| Rok vydání: | 2016 |
| Předmět: |
Glycosylamine
Stereochemistry Fimbria Disaccharide Stereoisomerism 010402 general chemistry 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Bacterial Adhesion Analytical Chemistry Mannans chemistry.chemical_compound Amide Carbohydrate Conformation Escherichia coli Mannobiose Organic chemistry Molecular Biology Fluorescent Dyes Carbodiimide 010405 organic chemistry Organic Chemistry Succinates General Medicine Microspheres 0104 chemical sciences Carbodiimides chemistry Fimbriae Bacterial Benzyl group Hydrogenation Mannose Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 80:128-134 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2015.1075863 |
| Popis: | Manα1→2Man, Manα1→3Man, Manα1→4Man, and Manα1→6Man were converted to the glycosylamine derivatives. Then, they were mixed with monobenzyl succinic acid to obtain their amide derivatives. After removing the benzyl group by hydrogenation, the succinylamide derivatives were coupled with the hydrazino groups on BlotGlyco™ beads in the presence of water-soluble carbodiimide. d-Mannobiose-linked beads were incubated with fluorescence-labeled Escherichia coli with type 1 fimbria, and the number of the fluorescent dots associated with the beads was counted in order to determine the binding preference among d-mannobiose isomers. The results showed that the bacteria bind strongly to Manα1→2Man1→beads, Manα1→3Man1→beads, Manα1→4Man1→beads, and Manα1→6Man1→beads, in order. In the presence of 0.1 M methyl α-d-mannopyranoside, most of the bacteria failed to bind to these beads. These results indicate that E. coli with type 1 fimbria binds to all types of d-mannobiose isomers but preferentially to Manα1→2Man disaccharide. |
| Databáze: | OpenAIRE |
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