Pressure effects on the chimeric 3-isopropylmalate dehydrogenases of the deep-sea piezophilic Shewanella benthica and the atmospheric pressure-adapted Shewanella oneidensis
| Autor: | Yuki Hamajima, Yasuyuki Kato-Yamada, Chiaki Kato, Nobuhisa Watanabe, Takeo Imai, Takayuki Nagae |
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| Rok vydání: | 2014 |
| Předmět: |
Shewanella
Shewanella benthica Recombinant Fusion Proteins Dehydrogenase 3-isopropylmalate Biology Applied Microbiology and Biotechnology Biochemistry Deep sea Analytical Chemistry 3-Isopropylmalate Dehydrogenase Catalytic Domain Pressure Seawater Shewanella oneidensis Molecular Biology chemistry.chemical_classification Atmospheric pressure Organic Chemistry General Medicine biology.organism_classification Enzyme chemistry Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 78:469-471 |
| ISSN: | 1347-6947 0916-8451 |
| Popis: | The chimeric 3-isopropylmalate dehydrogenase enzymes were constructed from the deep-sea piezophilic Shewanella benthica and the shallow water Shewanella oneidensis genes. The properties of the enzymatic activities under pressure conditions indicated that the central region, which contained the active center and the dimer forming domains, was shown to be the most important region for pressure tolerance in the deep-sea enzyme. |
| Databáze: | OpenAIRE |
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