Interactions between platelet-surface glycoproteins. Identification of glycoproteins capable of binding to platelet surfaces
| Autor: | D J Bowles, C Brunton |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Peanut agglutinin
Blood Platelets Glycoconjugate In Vitro Techniques Biochemistry Humans Platelet Receptor Molecular Biology Glycoproteins chemistry.chemical_classification biology Membrane Proteins Cell Biology Electrophoresis Membrane chemistry Galactose oxidase Receptors Mitogen biology.protein Microscopy Electron Scanning Electrophoresis Polyacrylamide Gel Glycoprotein Protein Binding Subcellular Fractions Research Article |
| Zdroj: | The Biochemical journal. 202(3) |
| ISSN: | 0264-6021 |
| Popis: | 1. Platelets have been isolated from plasma and their surface glycoconjugates radioactively-labelled using galactose oxidase and NaB3H4. 2. Conditions have been defined for optimal labelling of glycoproteins and a membrane fraction enriched in plasma membrane has been prepared and characterized by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. 3. Desialylated glycoproteins that act as receptors to peanut agglutinin and lentil lectin have been purified from a detergent extract of plasma membrane. 4. Two glycosylated polypeptides that are able to bind to the surfaces of platelets have been identified and some characteristics of the binding have been investigated. |
| Databáze: | OpenAIRE |
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