Control of initial endothelial spreading by topographic activation of focal adhesion kinase
| Autor: | Martin Bednarzik, Jens Gobrecht, Davide Franco, Dimos Poulikakos, Marco Cecchini, Mirko Klingauf, Aldo Ferrari, Vartan Kurtcuoglu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
0303 health sciences
Materials science biology business.industry Integrin 02 engineering and technology General Chemistry Adhesion 021001 nanoscience & nanotechnology Condensed Matter Physics Ridge (differential geometry) Nanoimprint lithography law.invention Endothelial stem cell Focal adhesion 03 medical and health sciences Optics law Cell polarity biology.protein Biophysics 0210 nano-technology business Groove (engineering) 030304 developmental biology |
| Zdroj: | Soft matter 7 (2011): 7313–7324. doi:10.1039/c1sm05191a info:cnr-pdr/source/autori:Franco D.; Klingauf M.; Bednarzik M.; Cecchini M.; Kurtcuoglu V.; Gobrecht J.; Poulikakos D.; Ferrari A./titolo:Control of initial endothelial spreading by topographic activation of focal adhesion kinase/doi:10.1039%2Fc1sm05191a/rivista:Soft matter (Print)/anno:2011/pagina_da:7313/pagina_a:7324/intervallo_pagine:7313–7324/volume:7 |
| DOI: | 10.1039/c1sm05191a |
| Popis: | The time required to re-establish a functioning endothelial cell layer after vascular implant placement is critical to the success of the respective cardiologic or surgical intervention. Topographic modifications of implant surfaces promise to expedite endothelial regeneration by triggering the activation of cellular machineries that facilitate cell spreading. Exploiting nanoimprint lithography techniques on cyclic olefin copolymer foils, we engineered biocompatible submicron- and micro-structured gratings with groove and ridge width of 1 or 5 m and groove depth ranging from 0.1 to 2 m. Our results reveal that both the onset of endothelial spreading and subsequent texture-guided cell polarization critically depend on the feature size of the underlying topography, yet are independently modulated by the surface texture. Specifically, we demonstrate that on gratings with ridge and groove width of 1 m and groove depth of 1 m or deeper, the onset of endothelial spreading is 40% faster than on flat substrates, and that the cells align within ten degrees to the gratings. On this topography, we identify two independently regulated phases: acceleration of the onset of spreading supported by the rapid activation of integrin signaling proceeding via Focal Adhesion Kinase, and contact guidance which requires ROCK1/2 and myosin-II dependent cell contractility and focal adhesion maturation. © 2011 The Royal Society of Chemistry. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|