Hyaluronic acid / chitosan multilayer coatings on neuronal implants for localized delivery of siRNA nanoplexes
| Autor: | Dieter Stoll, Martin Dauner, Michael Doser, Burkhard Schlosshauer, Ursula Mittnacht, Susanne Hossfeld, Hanna Hartmann, Rumen Krastev, Ana Paula Pêgo |
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| Rok vydání: | 2013 |
| Předmět: |
Cell Survival
Pharmaceutical Science Nanotechnology PC12 Cells Regenerative medicine Chitosan chemistry.chemical_compound Hyaluronic acid Cell Adhesion Animals Gene silencing Hyaluronic Acid RNA Small Interfering Cell adhesion Neurons Imidazoles technology industry and agriculture Prostheses and Implants Controlled release Nanostructures Rats chemistry Cell culture Gene Knockdown Techniques Drug delivery Biophysics rhoA GTP-Binding Protein |
| Zdroj: | Journal of Controlled Release. 168:289-297 |
| ISSN: | 0168-3659 |
| DOI: | 10.1016/j.jconrel.2013.03.026 |
| Popis: | Binding, stabilizing and promoting cellular uptake of siRNA are all critical efforts in creating matrices for the localized delivery of siRNA molecules to target cells. In this study, we describe the generation of chitosan imidazole/siRNA nanoplexes (NPs) embedded in nano scope polyelectrolyte multilayers (PEMs) composed of hyaluronic acid and chitosan for sustained and localized drug delivery. Regular PEM build-up, successful integration of NPs and controlled release under physiological conditions were shown. Biological efficacy was evaluated in neuronal cell culture concerning cell adhesion, viability, NPs uptake and gene silencing. The additionally shown biological functionalization of neuronal implants possesses potential for future applications in the field of regenerative medicine and treatment of spinal cord injuries. |
| Databáze: | OpenAIRE |
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