Effects of zinc on tissue uptake and toxicity of lead in Sprague Dawley rat
| Autor: | Yared B. Yohannes, Andrew Kataba, Haruya Toyomaki, Shouta M.M. Nakayama, Mayumi Ishizuka, Hokuto Nakata, Yoshinori Ikenaka |
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| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Antioxidant medicine.medical_treatment chemistry.chemical_element Apoptosis Zinc Toxicology medicine.disease_cause Antioxidants Rats Sprague-Dawley Internal medicine Testis medicine Animals oxidative stress lead Full Paper General Veterinary biology toxicity Enzyme assay Rats Endocrinology chemistry Lead acetate Catalase Toxicity biology.protein zinc co-administration Oxidative stress Peroxidase |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.20-0684 |
| Popis: | Lead (Pb) exposure occurs together with other metals including zinc (Zn). This study investigated the impact of Zn on Pb tissue accumulation and Pb-induced toxicities. Animals (n=6 rats per group) were exposed to lead acetate (PbAc) or a combination of PbAc and zinc acetate (ZnAc) under the following groups: control (deionized water), low PbAc [12 mg/kg PbAc (3 mg PbAc/rat/day)], low PbAc-ZnAc [12 mg/kg PbAc (3 mg PbAc/rat/day) + 0.2 mg ZnAc/rat/48 hr], high PbAc [120 mg/ kg ( 30 mg PbAc/rat/day)], and high PbAc- ZnAc [120 mg/ kg ( 0 mg PbAc/rat/ day) + 1 mg ZnAc/rat/48 hr] for 8 weeks. A significant reduction in body weight gain was observed in the high PbAc group relative to the control group. Muscles and testes both had reduced and increased Pb uptake in low PbAc-ZnAc and high PbAc-ZnAc groups compared to PbAc only groups, respectively. Bone Pb levels in the high PbAc-ZnAc group were lower than the high PbAc group. Zinc co-administration attenuated Pb-induced inhibition of delta aminolaevulinic acid dehydratase enzyme and enhanced catalase enzyme activity at a high level of exposure. Moreover, ZnAc seems to have minimized the effects of Pb-induced mRNA dysregulation in antioxidant and antiapoptotic enzymes encoding genes. Heme oxygenase- 1 was downregulated in the kidney and brain in the low PbAc group. Liver glutathione peroxidase and thioredoxin reductase-1 were downregulated in the high PbAc group. These findings suggest that zinc co-administration with lead may partially mitigate against Pb-induced toxicities. |
| Databáze: | OpenAIRE |
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