Fluoro-Jade® B staining as useful tool to identify activated microglia and astrocytes in a mouse transgenic model of Alzheimer's disease
Autor: | Jacques Hugon, Agnès Rioux Bilan, Milena Damjanac, Guylène Page, Cantereau Anne, Raymond Pontcharraud, Laurence Barrier |
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Přispěvatelé: | Institut du Fer à Moulin, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Groupe d'Etudes des Mécanismes Cellullaires de l'Ischémie, Partenaires INRAE, Cibles moléculaires et thérapeutiques de la maladie d'Alzheimer (CIMoTHeMA), Université de Poitiers |
Rok vydání: | 2007 |
Předmět: |
Genetically modified mouse
Pathology medicine.medical_specialty [SDV]Life Sciences [q-bio] Antigens Differentiation Myelomonocytic Mice Transgenic Transgenic Model Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Alzheimer Disease Antigens CD Glial Fibrillary Acidic Protein Presenilin-1 medicine Animals Humans DAPI Organic Chemicals Molecular Biology 030304 developmental biology 0303 health sciences Amyloid beta-Peptides biology Microglia Glial fibrillary acidic protein General Neuroscience Age Factors Brain Fluoresceins Immunohistochemistry Molecular biology Disease Models Animal medicine.anatomical_structure nervous system chemistry Astrocytes Phosphopyruvate Hydratase biology.protein Neuroglia Neurology (clinical) Amyloid Precursor Protein Secretases NeuN 030217 neurology & neurosurgery Developmental Biology Astrocyte |
Zdroj: | Brain Research Brain Research, Elsevier, 2006, 1128, pp.40-49. ⟨10.1016/j.brainres.2006.05.050⟩ |
ISSN: | 0006-8993 |
Popis: | International audience; Fluoro-Jade ® B is known as a high affinity fluorescent marker for the localization of neuronal degeneration during acute neuronal distress. However, one study suggested that fluoro-Jade ® B stains reactive astroglia in the primate cerebral cortex. In this study, we analyzed the staining of fluoro-Jade ® B alone or combined with specific markers for detection of glial fibrillary acidic protein (GFAP) or activated CD68 microglia in the double APP SL /PS1 KI transgenic mice of Alzheimer's disease (AD), which display a massive neuronal loss in the CA1 region of the hippocampus. Our results showed that fluoro-Jade ® B did not stain normal and degenerating neurons in this double mouse transgenic model. Fluoro-Jade ® B was colocalized with Aβ in the core of amyloid deposits and in glia-like cells expressing Aβ. Furthermore, fluoro-Jade ® B was co-localized with CD68/macrosialin, a specific marker of activated microglia, and with GFAP for astrocytes in APP SL /PS1 KI transgenic mice of AD. Taken together, these findings showed that fluoro-Jade ® B can be used to label activated microglia and astrocytes which are abundant in the brain of these AD transgenic mice. It could stain degenerating neurons as a result of acute insult while it could label activated microglia and astrocytes during a chronic neuronal degenerative process such as AD for example. |
Databáze: | OpenAIRE |
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