Fluoro-Jade® B staining as useful tool to identify activated microglia and astrocytes in a mouse transgenic model of Alzheimer's disease

Autor: Jacques Hugon, Agnès Rioux Bilan, Milena Damjanac, Guylène Page, Cantereau Anne, Raymond Pontcharraud, Laurence Barrier
Přispěvatelé: Institut du Fer à Moulin, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Groupe d'Etudes des Mécanismes Cellullaires de l'Ischémie, Partenaires INRAE, Cibles moléculaires et thérapeutiques de la maladie d'Alzheimer (CIMoTHeMA), Université de Poitiers
Rok vydání: 2007
Předmět:
Genetically modified mouse
Pathology
medicine.medical_specialty
[SDV]Life Sciences [q-bio]
Antigens
Differentiation
Myelomonocytic
Mice
Transgenic
Transgenic Model
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Alzheimer Disease
Antigens
CD
Glial Fibrillary Acidic Protein
Presenilin-1
medicine
Animals
Humans
DAPI
Organic Chemicals
Molecular Biology
030304 developmental biology
0303 health sciences
Amyloid beta-Peptides
biology
Microglia
Glial fibrillary acidic protein
General Neuroscience
Age Factors
Brain
Fluoresceins
Immunohistochemistry
Molecular biology
Disease Models
Animal
medicine.anatomical_structure
nervous system
chemistry
Astrocytes
Phosphopyruvate Hydratase
biology.protein
Neuroglia
Neurology (clinical)
Amyloid Precursor Protein Secretases
NeuN
030217 neurology & neurosurgery
Developmental Biology
Astrocyte
Zdroj: Brain Research
Brain Research, Elsevier, 2006, 1128, pp.40-49. ⟨10.1016/j.brainres.2006.05.050⟩
ISSN: 0006-8993
Popis: International audience; Fluoro-Jade ® B is known as a high affinity fluorescent marker for the localization of neuronal degeneration during acute neuronal distress. However, one study suggested that fluoro-Jade ® B stains reactive astroglia in the primate cerebral cortex. In this study, we analyzed the staining of fluoro-Jade ® B alone or combined with specific markers for detection of glial fibrillary acidic protein (GFAP) or activated CD68 microglia in the double APP SL /PS1 KI transgenic mice of Alzheimer's disease (AD), which display a massive neuronal loss in the CA1 region of the hippocampus. Our results showed that fluoro-Jade ® B did not stain normal and degenerating neurons in this double mouse transgenic model. Fluoro-Jade ® B was colocalized with Aβ in the core of amyloid deposits and in glia-like cells expressing Aβ. Furthermore, fluoro-Jade ® B was co-localized with CD68/macrosialin, a specific marker of activated microglia, and with GFAP for astrocytes in APP SL /PS1 KI transgenic mice of AD. Taken together, these findings showed that fluoro-Jade ® B can be used to label activated microglia and astrocytes which are abundant in the brain of these AD transgenic mice. It could stain degenerating neurons as a result of acute insult while it could label activated microglia and astrocytes during a chronic neuronal degenerative process such as AD for example.
Databáze: OpenAIRE
Externí odkaz: