The Potential of IgG to Induce Murine and Human Thymic Maturation of IL-10+ B Cells (B10) Revealed in a Pilot Study
| Autor: | Marília Garcia de-Oliveira, Fábio da Ressureição Sgnotto, Amanda Harumi Sabô Inoue, Thamires Rodrigues de Sousa, Alberto José da Silva Duarte, Jefferson Russo Victor, Aline Aparecida de Lima Lira |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
0301 basic medicine Allergy IgG Pilot Projects Spleen chemical and pharmacologic phenomena macromolecular substances Peripheral blood mononuclear cell Article Mice 03 medical and health sciences 0302 clinical medicine thymus medicine Animals Humans human lcsh:QH301-705.5 mouse B-Lymphocytes B cells biology fungi food and beverages hemic and immune systems General Medicine medicine.disease In vitro Interleukin-10 Disease Models Animal Ovalbumin Interleukin 10 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) B10 cells Immunoglobulin G CD1D Immunology biology.protein Female Bone marrow 030215 immunology |
| Zdroj: | Cells Volume 9 Issue 10 Cells, Vol 9, Iss 2239, p 2239 (2020) |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells9102239 |
| Popis: | Regulatory B (B10) cells can control several inflammatory diseases, including allergies however, the origin of peripheral B10 cells is not fully understood, and the involvement of primary lymphoid organs (PLOs) as a primary site of maturation is not known. Here, using a murine model of allergy inhibition mediated by maternal immunization with ovalbumin (OVA), we aimed to evaluate whether B10 cells can mature in the thymus and whether IgG can mediate this process. Female mice were immunized with OVA, and offspring thymus, bone marrow, spleen, lung, and serum samples were evaluated at different times and after passive transfer of purified IgG or thymocytes. A translational approach was implemented using human nonatopic thymus samples, nonatopic peripheral blood mononuclear cells (PBMCs), and IgG from atopic or nonatopic individuals. Based on the expression of CD1d on B cells during maturation stages, we suggest that B10 cells can also mature in the murine thymus. Murine thymic B10 cells can be induced in vitro and in vivo by IgG and be detected in the spleen and lungs in response to an allergen challenge. Like IgG from atopic individuals, human IgG from nonatopic individuals can induce B10 cells in the infant thymus and adult PBMCs. Our observations suggest that B10 cells may mature in the thymus and that this mechanism may be mediated by IgG in both humans and mice. These observations may support the future development of IgG-based immunoregulatory therapeutic strategies. |
| Databáze: | OpenAIRE |
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