Integrated K+ channel and K+Cl- cotransporter functions are required for the coordination of size and proportion during development
| Autor: | Caroline Stanclift, Jennifer Lanni, Matthew P. Harris, Kristopher T. Kahle, Haining Chen, Gerardo Gamba, Margot E. Bowen, Adriana Mercado, David Peal, Laura J. Ekstrom |
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| Rok vydání: | 2019 |
| Předmět: |
Male
animal structures Mutant Article Potassium Chloride 03 medical and health sciences 0302 clinical medicine Missense mutation Animals Regeneration Molecular Biology Zebrafish Loss function 030304 developmental biology Cell Size 0303 health sciences biology Symporters Cell Biology Organ Size Zebrafish Proteins biology.organism_classification Phenotype Potassium channel Cell biology Potassium Channels Voltage-Gated embryonic structures Mutation Animal Fins Female Signal transduction Cotransporter 030217 neurology & neurosurgery Developmental Biology Signal Transduction |
| Zdroj: | Dev Biol |
| ISSN: | 1095-564X |
| Popis: | The coordination of growth during development establishes proportionality within and among the different anatomic structures of organisms. Innate memory of this proportionality is preserved, as shown in the ability of regenerating structures to return to their original size. Although the regulation of this coordination is incompletely understood, mutant analyses of zebrafish with long-finned phenotypes have uncovered important roles for bioelectric signaling in modulating growth and size of the fins and barbs. To date, long-finned mutants identified are caused by hypermorphic mutations, leaving unresolved whether such signaling is required for normal development. We isolated a new zebrafish mutant, schleier, with proportional overgrowth phenotypes caused by a missense mutation and loss of function in the K(+)-Cl(−) cotransporter Kcc4a. Creation of dominant negative Kcc4a in wild-type fish leads to loss of growth restriction in fins and barbs, supporting a requirement for Kcc4a in regulation of proportion. Epistasis experiments suggest that Kcc4a and the two-pore potassium channel Kcnk5b both contribute to a common bioelectrical signaling response in the fin. These data suggest that an integrated bioelectric signaling pathway is required for the coordination of size and proportion during development. |
| Databáze: | OpenAIRE |
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