Usefulness of capillary electrophoresis-based multiplex PCR assay for species-specific identification of Candida spp
| Autor: | Francesca Mallus, Tania Camboni, Corrado Serra, Aldo Manzin, S Martis, G Loi |
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| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Microbiological Techniques Time Factors Electrophoresis Capillary Context (language use) Mycology Biology Microbiology Molecular biology Capillary electrophoresis Multiplex polymerase chain reaction Candida spp Humans Multiplex Molecular Biology Multiplex Polymerase Chain Reaction Specific identification Candida |
| Zdroj: | Journal of microbiological methods. 92(2) |
| ISSN: | 1872-8359 |
| Popis: | The study evaluated the performances of a commercial multiplex PCR assay, the Seegene Seeplex STI Master Panel 3, for Candida spp. identification. Eighty clinical strains of Candida spp. were identified with this system and a homemade multiplex PCR assay. The results were also compared with those obtained with two phenotypic methods. The study provided a preliminary evaluation of a multiplex assay from Seegene that uses capillary electrophoresis as the detection of amplified products. The Seeplex assay was found to be a rapid and useful method for identifying large numbers of yeast isolates in the clinical laboratory context. |
| Databáze: | OpenAIRE |
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